Published August 26, 2024 | Version v1
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Morphological and molecular data warrant the description of a new species of the genus Scutiger (Anura, Megophryidae) from the Central Himalaya

  • 1. Leibniz Institute for the Analysis of Biodiversity Change, Museum Koenig, Bonn, Germany|Helmholtz-Centre for Environmental Research GmbH – UFZ, Department Conservation Biology, Leipzig, Germany
  • 2. Comenius University in Bratislava, Bratislava, Slovakia
  • 3. University of Rostock, Rostock, Germany

Description

Recent phylogenetic studies in Himalayan lazy toads of the genus Scutiger Theobald, 1868 revealed the presence of genetically deeply divergent lineages. The taxonomy of S. nepalensis sensu lato was re-assessed based on museum material considering molecular and morphological data. The results strongly support the recognition of a new species, S. kanjiroba sp. nov. distributed along the Nepalese Kanjiroba massif. It is further shown that S. sikimmensis has an apparently much more restricted distribution range than previously thought. The frequent misidentification of Scutiger across large areas of the Himalaya-Tibet area highlights the significance of careful taxonomic evaluation of collection material and the need for the direct morphological comparison of closely related species when describing new species.

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References

  • Agarwal I, Bauer AM, Jackman TR, Karanth KP (2014) Insights into Himalayan biogeography from geckos: A molecular phylogeny of Cyrtodactylus (Squamata: Gekkonidae). Molecular Phylogenetics and Evolution 80: 145–155. https://doi.org/10.1016/j.ympev.2014.07.018
  • Bryant D, Moulton V (2004) Neighbor-net: An agglomerative method for the construction of phylogenetic networks. Molecular Biology and Evolution 21(2): 255–265. https://doi.org/10.1093/molbev/msh018
  • Dubois A (1987) Miscellanea taxinomica batrachologica (I). Alytes 5: 7–95 (1986). https://www.biodiversitylibrary.org/page/62924166#page/9/mode/1up
  • Dubois A (1995) Keratodont formulae in anuran tadpoles: Proposal for standardisation. Journal of Zoological Systematics and Evolutionary Research 33: 1–15. https://doi.org/10.1111/j.1439-0469.1995.tb00207.x
  • Fei L, Ye C-y (2016) Amphibians of China, Volume 1. Chengdu Institute of Biology, Chinese Academy of Sciences, Science Press, Beijing, China, 461 pp.
  • Fei L, Hu S-q, Huang Y-z (2009) Fauna Sinica. Amphibia. Volume 2. Anura. Chinese Academy of Science. Science Press, Beijing, 957 pp.
  • Fei L, Ye CJ, Jiang JP (2012) Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Science and Technology Press, Sichuan, China, 620 pp.
  • Frost DR (2024) Amphibian species of the world: an online reference. Version 6.2. https://amphibiansoftheworld.amnh.org/index.php [accessed 19 May 2024]
  • Gosner KL (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16: 183–190. https://www.jstor.org/stable/3890061
  • Hofmann S, Stoeck M, Zheng Y, Ficetola FG, Li JT, Scheidt U, Schmidt J (2017) Molecular Phylogenies indicate a Paleo-Tibetan Origin of Himalayan Lazy Toads (Scutiger). Scientific Reports 7(1): 3308. https://doi.org/10.1038/s41598-017-03395-4
  • Hofmann S, Podsiadlowski L, Andermann T, Matschiner M, Baniya CB, Litvinchuk SN, Martin S, Masroor R, Yang J, Zheng Y, Jablonski D, Schmidt J (2024) The last of their kind: Is the genus Scutiger (Anura: Megophryidae) a relict element of the paleo-transhimalaya biota? Molecular Phylogenetics and Evolution 2024: 108166. https://doi.org/10.1016/j.ympev.2024.108166
  • Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution 23(2): 254–267. https://doi.org/10.1093/molbev/msj030
  • Jetz W, McPherson JM, Guralnick RP (2012) Integrating biodiversity distribution knowledge: Toward a global map of life. Trends in Ecology & Evolution 27(3): 151–159. https://doi.org/10.1016/j.tree.2011.09.007
  • Kattel GR (2022) Climate warming in the Himalayas threatens biodiversity, ecosystem functioning and ecosystem services in the 21st century: Is there a better solution? Biodiversity and Conservation 31(8–9): 2017–2044. https://doi.org/10.1007/s10531-022-02417-6
  • Khatiwada JR, Shu GC, Subedi TR, Wang B, Ohler A, Canatella DC, Xie F, Jiang JP (2019) A new species of megophryid frog of the genus Scutiger from Kangchenjunga Conservation Area, eastern Nepal. Asian Herpetological Research 10: 139–157. https://archive.org/details/ahr-20190301
  • Le S, Josse J, Husson F (2008) FactoMineR: An R Package for Multivariate Analysis. Journal of Statistical Software 25(1): 1–18. https://doi.org/10.18637/jss.v025.i01
  • Mittermeier RA, Turner WR, Larsen FW, Brooks TM, Gascon C (2011) Global Biodiversity Conservation: The Critical Role of Hotspots In: Zachos FE, Habel JC (Eds) Biodiversity Hotspots. Springer, Berlin / Heidelberg, 3–22. https://doi.org/10.1007/978-3-642-20992-5_1
  • Myers GS, Leviton AE (1962) Generic Classification of the High-Altitude Pelobatid Toads of Asia (Scutiger, Aelurophryne, and Oreolalax). Copeia 1962(2): 287–291. https://doi.org/10.2307/1440892
  • Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GA, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403(6772): 853–858. https://doi.org/10.1038/35002501
  • Pandit MK (2013) The Himalayas must be protected. Nature 501(7467): 283. https://doi.org/10.1038/501283a
  • Paudel PK, Sipos J, Brodie JF (2018) Threatened species richness along a Himalayan elevational gradient: Quantifying the influences of human population density, range size, and geometric constraints. BMC Ecology 18(1): 6. https://doi.org/10.1186/s12898-018-0162-3
  • Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA (2018) Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7. Systematic Biology 67(5): 901–904. https://doi.org/10.1093/sysbio/syy032
  • RCoreTeam (2024) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org [accessed 26 April 2024]
  • Schleich HH, Kästle W (2002) Amphibians and Reptiles of Nepal. A.R.G. Gantner Verlag, Ruggell, 1200 pp.
  • Schmidt J, Opgenoorth L, Miehe G (2016) Speciation, uplift, and climate change. In: Miehe G, Pendry C (Eds) Nepal. An introduction to the natural history, ecology and human environment in the Himalayas. A companion to the Flora of Nepal. Royal Botanic Garden Edinburgh, Edinburgh, 168–173.
  • Schmidt J, Opgenoorth L, Mao K, Baniya CB, Hofmann S (2023) Molecular phylogeny of mega-diverse Carabus attests late Miocene evolution of alpine environments in the Himalayan-Tibetan Orogen. Scientific Reports 13(1): 13272. https://doi.org/10.1038/s41598-023-38999-6
  • Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022–3027. https://doi.org/10.1093/molbev/msab120
  • Wickham H (2016) ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag, New York, 260 pp. https://link.springer.com/chapter/10.1007/978-3-319-24277-4_9