Published August 12, 2024 | Version v1

SAXS Data of Phospholipid Mixtures forming the Inverted Hexagonal Phase

  • 1. ROR icon University of Graz
  • 1. Karl-Franzens-Universität Graz
  • 2. ROR icon University of Graz

Description

Lipids and Experimental Info

Methods

  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)
  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE)
  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPE)

Tricosene (12 wt%) was added to remove stress from the monolayers

Technical info

SAXS experiments were performed using a SAXSpoint5.0 camera (Anton Paar, Graz, Austria) connected to a MetalJet (Excillum, Kista, Sweden) with a liquid, Ga-rich alloy jet anode. This system was equipped with an Eiger R1 M detector (Dectris, Baden-
Daettwil, Switzerland). Samples were contained in paste cells (Anton Paar) and exposed to X-rays for  2 minutes (12~frames \`{a} 10~s). All samples were equilibrated for 10~minutes at 35°C (TC 150, Anton Paar) for 10 minutes prior to measurement. The sample-to-detector distance was 700 mm. Data were background corrected and integrated using SAXSanalysis (Anton Paar).

Files

SAXS_Data.zip

Files (330.9 kB)

Name Size Download all
md5:7c52b81b8dc6cd10b222dde5484bec74
330.9 kB Preview Download

Additional details

Funding

FWF Austrian Science Fund
Effect of Lipid Asymmetry on the Enzymatic Activity of OmpLA 10.55776/P32514