Published August 1, 2018 | Version v1
Dataset Open

Purification of Recombinant Cas9

Description

SGC Open Notebook Project to Characterize the HMTase NSD3

Exp027 Objective: To purify recombinant Cas9 protein for genome editing experiments. Recombinant Cas9 will be used with synthesized crRNA:tracrRNA to assemble ribonucleoproteins (RNPs) to target the nuclease activity of Cas9 to specific loci within the genome. Using RNPs as opposed to viral delivery of CRISPR gene editing components is advantageous as the expression of Cas9 is transient limiting off-target effects. 

Notes

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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Cas9Prep_01082018_exp27.pdf

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