Published July 11, 2018 | Version v1
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PHYTO-SYNTHESIZED SILVER NANOPARTICLES: A POTENT MOSQUITO OVICIDAL AGENT

Authors/Creators

  • 1. Unit of Vector control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.

Description

Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and eco-friendly reducing and capping agents. The present study to determine the efficacies of synthesized silver nanoparticles (AgNPs) using aqueous leaf extract of Feronia elephantum against the eggs of malaria vector, Anopheles stephensi , dengue vector, Aedes aegypti and filariasis vector, Culex quinquefasciatus. Eggs were exposed to varying concentrations of aqueous extract of F.elephantum and synthesized Ag NP for 24 h. Ag NP were rapidly synthesized using the leaf extract of F.elephantum and the formation of nanoparticles was observed within 6 h. After treatment, the eggs from each concentration were individually transferred to distilled water cups for hatching assessment after counting the eggs under microscope. Each experiment was replicated six times along with appropriate control. The hatch rates were assessed 48 h post – treatment. The aqueous leaf extract and AgNPs exerted 100% mortality (zero hatchability-12 to 18 hrs eggs) at 250, 300 and 350 μg/mL, and 60, 70 and 80 μg/mL, against An. stephensi, Ae.aegypti and Cx. quinquefasciatus, respectively. The results recorded from UV - vis spectrum, Fourier transform infrared, X-ray diffraction (XRD) analysis. Scanning electron microscopy and transmission electron microscopy support the biosynthesis and characterization of AgNPs. This is the first report on ovicidal activity of the plant extract and synthesized AgNPs.

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References

  • Ali, A., Nayar, J.K. and Xue, R.D., 1995. Comparative toxicity of selected larvicides and insect growth regulators to a Florida laboratory, population of Aedes albopictus. J. Am. Mosq. Control. Assoc., 11(1), 72-76.
  • Arjunan, N.K., Murugan, K., Rejeeth, C., Madhiyazhagan, P. and Barnard, D.R., 2012. Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue. Vector-Borne Zoonotic Dis., 12(3), 262-268.
  • Benelli, G., Conti, B., Garreffa, R. and Nicoletti, M., 2014. Shedding light on bioactivity of botanical by-products: neem cake compounds deter oviposition of the arbovirus vector Aedes albopictus (Diptera: Culicidae) in the field. Parasitol. Res., 113, 933-940.
  • Benn, T. and Westerhoff, P., 2008. Nanoparticle silver released into water from commercially available sock fabrics. Environ. Sci. Technol., 42, 4133-4139.
  • Bernhard, L., Bernhard, P. and Magnussen, P., 2003. Management of patients with lymphoedema caused by filariasis in northeastern Tanzania: alternative approaches. Physiotherapy, 89, 743-749.
  • Chandre, F., Darriet, F., Darder, M., Cuany, A., Doannio, J.M.C., Pasteur, N. and Guillet, P., 1998. Pyrethroid resistance in Culex quinquefasciatus from West Africa. Med. Vet. Entomol., 12, 359-366.
  • Das, P.K., Pani, S.P. and Krishnamoorthy, K., 2000. Prospects of elimination of lymphatic filariasis in India. ICMR Bull 32(5-6), 41-54.
  • Govindarajan, M., 2010a. Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (willd.) Hook. F. Benth (Rutaceae) against three mosquito species. Asian Pacific J. Trop.Med., 3, 874-877.
  • Govindarajan, M., 2010b. Larvicidal efficacy of Ficus benghalensis L. plant leaf extracts against Culex quinquefasciatus Say, Aedes aegypti L. and Anopheles stephensi L. (Diptera: Culicidae). Eur. Rev. Med. Pharmacol. Sci., 14(2), 107-111.
  • Govindarajan, M. and Sivakumar, R., 2012. Adulticidal and repellent properties of indigenous plant extracts against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Parasitol. Res., 110, 1607-1620.
  • Govindarajan, M., Jebanesan, A., Pushpanathan, T. and Samidurai, K., 2008. Studies on effect of Acalypha indica L. (Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res., 103(3), 691-695.
  • Govindarajan, M., Rajeswary, M., Hoti, S.L., Murugan, K., Kovendan, K., Arivoli, S. and Benelli, G. 2016a. Clerodendrum chinense-mediated biofabrication of silver nanoparticles: Mosquitocidal potential and acute toxicity against non-target aquatic organisms. J. Asia-Pacific Entomol., 19(1), 51-58.
  • Govindarajan, M., Rajeswary, M., Veerakumar, K., Muthukumaran, U., Hoti, S.L. and Giovanni Benellic, 2016b. Green synthesis and characterization of silver nanoparticles fabricated using Anisomeles indica: Mosquitocidal potential against malaria, dengue and Japanese encephalitis vectors. Exper. Parasitol., 161, 40-47.
  • Green, M.M., Singer, J.M. Sutherland, D.J. and Hibben, C.R., 1991. Larvicidal activity of Tagetes minuta (Marigold) toward Aedes aegypti. J. Amer. Mosq. Control. Ass., 7, 282-286.
  • Kamakshi, K.T., Raveen, R., Tennyson, S., Arivoli, S. and Reegan, D.A., 2015. Ovicidal and repellent activities of Cereus hildmannianus (K.Chum.) (Cactaceae) extracts against the dengue vector Aedes aegypti L. (Diptera: Culicidae). Int. J. Mosq. Res., 2(1), 13-17.
  • Kovendan, K., Murugan, K., Vincent, S. and Barnard, D.R., 2012. Studies on larvicidal and pupicidal activity of Leucas aspera Willd. (Lamiaceae) and bacterial insecticide, Bacillus sphaericus against malarial vector, Anopheles stephensi Liston. (Diptera: Culicidae). Parasitol. Res., 110, 195-203
  • Marimuthu, S., Rahuman, A.A., Rajakumar, G., Santhoshkumar, T., Kirthi, A.V., Jayaseelan, C., Bagavan, A., Zahir, A.A., Elango, G., Kamaraj, C., 2010. Evaluation of green synthesized silver nanoparticles against parasites. Parasitol. Res., 108(6), 1541-1549.
  • Muthukumaran, U., Govindarajan, M., Rajeswary, M., Hoti, S.L., 2014. Synthesis and characterization of silver nanoparticles using Gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes. Parasitol Res., 114, 1817-1827.
  • Naresh Kumar, A., Murugan, K., Madhiyazhagan, P., Kovendan, K., Prasannakumar, K., Thangamani, S. and Barnard, D., 2011. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus. Parasitol. Res., 110(4), 1435-43.
  • Panneerselvam, C., Ponarulselvam, S. and Murugan, K. 2011. Potential antiplasmodial activity of synthesized silver nanoparticle using Andrographis paniculata Nees (Acanthaceae). Arch. Appl. Sci. Res., 3(6), 208-217.
  • Rajkumar, G. and Abdul Rahuman, A. 2011. Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta. Trop., 118(3), 196-203.
  • Reegan, A.D., Gandhi, M.R., Paulraj, M.G., Balakrishna, K. and Ignacimuthu, S. 2014. Effect of niloticin, a protolimonoid isolated from Limonia acidissima L. (Rutaceae) on the immature stages of dengue vector Aedes aegypti L. (Diptera: Culicidae). Acta. Trop., 139, 67-76.
  • Salunkhe, R.B., Patil, S.V., Patil, C.D. and Salunke, B.K., 2011. Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res., 109(3), 823-831.
  • Sharma, P., Mohan, L. and Srivastava, C.N., 2009. Amaranthus oleracea and Euphorbia hirta: natural potential larvicidal agents against the urban Indian malaria vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res. 106, 171-176.
  • Su, T. and Mulla, M.S., 1998. Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J. Am. Mosq. Cont. Ass., 14, 204-209.
  • Subarani, S., Sabhanayakam, S. and Kamaraj, C., 2013. Studies on the impact of biosynthesized silver nanoparticles (AgNPs) in relation to malaria and filariasis vector control against Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol. Res., 112, 487-499.
  • Veerakumar, K., Govindarajan, M. and Rajeswary, M., 2013. Green synthesis of silver nanoparticles using Sida acuta (Malvaceae) leaf extract against Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti (Diptera: Culicidae). Parasitol. Res., 112, 4073-4085.
  • Veerakumar, K., Govindarajan, M. and Hoti, S.L., 2014a. Adulticidal activity of silver nanoparticles (AgNPs) synthesized Using Heliotropium indicum plant leaf extract against adults of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. Parasitol. Res.,113(12), 4567-77. doi: 10.1007/s00436-014-4147-7.
  • Veerakumar, K., Govindarajan, M. and Rajeswary, M., 2014b. Mosquito larvicidal properties of silver nanoparticles synthesized using Heliotropium indicum (Boraginaceae) against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae). Parasitol. Res., 113, 2363-2373.
  • Veerakumar, K., Govindarajan, M. and Hoti, S.L., 2014c. Evaluation of plant-mediated synthesized silver nanoparticles against vector mosquitoes. Parasitol Res., 113:4567-4577.
  • World Health Organisation, 2010. Malaria. Factsheet No.94.Geneva.http://www.who.int/mediacentre/factsheets/ fs094/en/ [Accessed July 2010].
  • Yamar, B.A., Diallo, D., Kebe, C.M., Dia, I. and Diallo, M., 2005. Aspects of bioecology of two Rift valley fever virus vectors in Senegal (West Africa) Aedes vexanus and Culex poicilipes (Diptera; Culicidae). J. Med. Entomol., 42(5), 739-750.

Subjects

Zoology
10.5281/zenodo.1309397