Published June 11, 2018 | Version v1
Dataset Open

Construct design for the crystallisation of huntingtin fragments - 2018/06/11

Authors/Creators

  • 1. Structural Genomics Consortium, University of Toronto

Description

Project - Huntingtin structure-function open lab notebook. 

Objective - Use the cryo-EM structure of huntingtin in complex with HAP40 http://www.rcsb.org/structure/6EZ8 to guide construct design of discrete domains and fragments which could be amenable to successful expression and purification of monodisperse protein samples and subsequent structure solution by X-ray crystallography. 

 

Notes

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. This project is funded and supported with generous funding from the Huntington Society of Canada and the CHDI Foundation. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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Construct design for the crystallisation of huntingtin fragments 2018:06:11.pdf

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