The TCR assigns naive T cells to a preferred lymph node
Authors/Creators
- 1. Utrecht University
- 2. Paul Ehrlich Institut
- 3. University of Paris
- 4. Aix-Marseille University
- 5. Grenoble Alpes University
Description
Naive T cells recirculate between the spleen and lymph nodes where they mount immune responses when meeting dendritic cells presenting foreign antigen. As this may happen anywhere, naive T cells ought to visit all lymph nodes. Here, deep sequencing almost-complete TCR-repertoires led to a comparison of different lymph nodes within and between individual mice. We find strong evidence for a deterministic CD4/CD8 lineage choice and a consistent spatial structure. Specifically, some T cells show a preference for one or multiple lymph nodes, suggesting that their TCR interacts with locally presented (self-)peptides. These findings are mirrored in TCR-transgenic mice showing localized CD69-expression, retention, and cell division. Thus, naïve T cells intermittently sense antigenically dissimilar niches, which is expected to affect their homeostatic competition.
Notes
Funding provided by: Deutsche Forschungsgemeinschaft
ROR ID: https://ror.org/018mejw64
Award Number: FOR1961 Ki611/2-1
Funding provided by: Dutch Research Council
ROR ID: https://ror.org/04jsz6e67
Award Number: 022.005.023
Funding provided by: Deutsche Forschungsgemeinschaft
ROR ID: https://ror.org/018mejw64
Award Number: Ki611/2-2