Published May 23, 2018
| Version v2
Dataset
Open
Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells
Description
SGC Open Notebook Project to Characterize the HMTase NSD3
Exp023 Objective: Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting.
Notes
Files
exp023.pdf
Files
(8.6 MB)
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