Extracted sequences of fungal OTUs on coarse woody debris Bavarian Forest

Extarcted sequences of fungal communities in coarse woody debris, sampled in the Bavarain Forest, Germany, in 2012, 2013 and 2015. A deadwood experiment with 67 stems (length 5m, dia ~33cm; 34x Fagus sylvatica and 33x Abies alba) was established in 2011. Per sampling year four drilling cores per stem were collected and pooled. PCR was performed on ITS2 region using barcoded gITS7 and ITS4. Treatment describes whether it is an experimentally created forest gap or a closed canopy. Taxonomic information and community composition only references to the most abundant OTUs used in Rieker et al. (2024): How to best detect threatened deadwood fungi – comparing metabarcoding and fruit body surveys. Biological Conservation.

Sample collection and processing are further described in

Rieker et al. (2024): How to best detect threatened deadwood fungi – comparing metabarcoding and fruit body surveys. Biological Conservation.

and lab protocols follow Baldrian et al. (2016): Fungi associated with decomposing deadwood in a natural beech-dominated forest. Fungal ecology, 23, 109-122.