Scanning electron microscopy datasets -- Emiliania huxleyi strains from naturally high and low CO2 waters responding to high and low CO2 in the lab

Study question: How do Emiliania huxleyi strains isolated from naturally high CO2 waters or low CO2 waters respond to exposure to high and low CO2 levels?

Associated article:
Peter von Dassow, Francisco Díaz-Rosas, El Mahdi Bendif, Juan-Diego Gaitán-Espitia, Daniella Mella-Flores, Sebastian Rokitta, Uwe John, and Rodrigo Torres. 2018. Over-calcified forms of the coccolithophore Emiliania huxleyi in high-CO2 waters are not preadapted to ocean acidification. Biogeosciences. https://doi.org/10.5194/bg-15-1-2018

Technical notes:

Three electron microscopes were used:

1. TM3000 (Hitachi High-Technologies, Tokyo, Japan) in the Unidad de Microscopía Avanzada of the Facultad de Ciencias Biológicas, Pontificia Univesidad Católica de Chile. The Hitachi microscope is not of high quality, and, when available, other electron microscopes were used.
2. Quanta 250 (FEI, Hillsboro, Oregon, USA) in the Facultad de Geología, Universidad de Chile
3. Quanta FEG 250 (FEI, Hillsboro, Oregon, USA) in the laboratory CIEN-UC, Facultad de Física, Pontificia Universidad Católica de Chile.

Data set 1: Data-sharing-SEM_Calfuco-CO2 experiment.zip

Scanning electron microscopy images of E. huxleyi strains after bubbling with 1200 µatm CO2 and 400 µatm CO2 air/CO2 mixes.

Data set 2: Field-SEM-2011-2013.zip

Scanning electron microscope images of filters of plankton samples taken during field campaigns. See article for methodology. For the samples from ElQuisco_2012 and JuanFernandez_2011, note that the last two digits in the sample name refer to the depth from which the sample was obtained (ej., “FQ.01.01.05D” is from 5 m and “FQ.01.01.15D” is from 15 m).  Tables are provided to associate counts and taxonomic identifications to environmental variables from the samples for which data was used in statistical analysis. Note also that images do not correspond to all counts reported, as sometimes counts were made without capturing images due to time pressure for microscope use.