Mapping of theCYP2JCytochrome P450 Genes to Human Chromosome 1 and Mouse Chromosome 4

CYP2J subfamily cytochromes P450 catalyze the NADPH-dependent oxidation of arachidonic acid to several unique eicosanoids that possess numerous biological activities including modulation of ion transport, control of bronchial and vascular smooth muscle tone, and stimulation of peptide hormone secretion. We have identified sequence variants in the 3′ untranslated regions of two mouseCyp2jgenes (Cyp2j5andCyp2j6) and used a PCR-based oligonucleotide hybridization assay to map both genes to the central region of chromosome 4 distal to theJunoncogene. The corresponding humanCYP2Jgene (CYP2J2) has been assigned to human chromosome 1 on a panel of somatic hybrid cell lines and to 1p31.3–p31.2 by fluorescencein situhybridization analysis. The proximity of theCyp2jcluster to theCyp4acluster suggests that these genes may be part of a cassette of P450 genes involved in the oxidation of fatty acids.