Journal article Open Access

Fluorescence in Situ Hybridization Mapping of Human Chromosome 19: Cytogenetic Band Location of 540 Cosmids and 70 Genes or DNA Markers

Trask, Barbara; Fertitta, Anne; Christensen, Mari; Youngblom, Janey; Bergmann, Anne; Copeland, Alex; de Jong, Pieter; Mohrenweiser, Harvey; Olsen, Anne; Carrano, Anthony; Tynan, Katherine

We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers.

Files (873.6 kB)
Name Size
article.pdf
md5:2e435dd9b1b3f3eba0592d2d31658352
873.6 kB Download
63
69
views
downloads
Views 63
Downloads 69
Data volume 60.3 MB
Unique views 61
Unique downloads 59

Share

Cite as