Journal article Open Access

Coaxial continuous flow fast atom bombardment for higher-molecular-weight peptides: Comparison with static fast atom bombardment and electrospray ionization

Tomer, Kenneth B.; Perkins, John R.; Parker, Carol E.; Deterding, Leesa J.

A comparison of coaxial continuous flow fast atom bombardment (FAB) with static FAB and with electrospray ionization (ESI) for the analysis of 'high'‐mass peptides (Mr = 3000–4000) is presented. Sensitivities of the peptides by coaxial continuous flow FAB is nearly an order of magnitude better than by static FAB. Single‐scan spectra with good signal‐to‐noise can be obtained from as little as 200 fmol (by flow injection analysis). Detection limits by ESI mass spectrometry were found to be equivalent to 20 times higher than by coaxial continuous flow FAB on a per mole basis, but 4–20 times lower on a concentration basis, owing to the greater flow per unit time employed in the ESI mass spectrometric experiments.

Files (437.6 kB)
Name Size
article.pdf
md5:0c6d8798daec113c44c5ed051898c835
437.6 kB Download
74
47
views
downloads
Views 74
Downloads 47
Data volume 20.6 MB
Unique views 74
Unique downloads 47

Share

Cite as