Published April 22, 2018
| Version v1
Dataset
Open
Wound Healing Assays in NSD3short Overexpressing H1299 Cells
Description
SGC Open Notebook Project to Characterize the HMTase NSD3
Exp022 Objective: During epithelial to mesenchymal transitioning (EMT), transcription networks become activated and cytoskeletal rearrangements take place. These events promote migratory capacity and invasiveness. A common assay to measure this phenotype is wound healing. This involves plating cells and culturing until confluent at which point a scratch or wound is made in the culture. This area is imaged over a 24-72 period to monitor the rate of wound closure. Cells with increased migratory capacity will close the wound faster and are indicative of a more mesenchymal state
Notes
Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Files
exp022.pdf
Files
(1.6 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:6be43898b6df7346a656b998d25647d1
|
1.6 MB | Preview Download |