Published June 22, 2024 | Version Anacapa Version 1, Crux reference databases Version 2.
Dataset Open

Soil Biome Variation of Lupinus nipomensis in Wet-Cool vs. Dry-Warm Microhabitats and Greenhouse Conditions

Authors/Creators

  • 1. ROR icon University of California, Santa Cruz
  • 2. University of California Santa Cruz
  • 3. ROR icon University of Hawaii System
  • 4. ROR icon University of California, Santa Barbara

Description

This is an eDNA metabarcoding dataset for four markers associated with the manscript about Lupinus nipomensis bulk soil eDNA from wet-cool and dry-warm habitats in natural areas and in greenhouse experiments. The same authors on that manuscript are included here. The manuscript is published in the American Journal of Botany

Methods (English)

We used the CALeDNA methods to generate multilocus metabarcoding libraries targeting the ITS1 (fungal DNA), CO1 (invertebrates and protists), 18S (eukaryotes), and 16S (bacterial and archaeal) regions. Soil samples were collected from greenhouse-grown Lupinus nipomensis individuals and from experimental field plots representing wet-cool and dry-warm microhabitats. DNA was extracted using the Qiagen DNeasy PowerSoil kit, amplified with Nextera-adapted primers, and sequenced on an Illumina MiSeq with a target depth of 50,000 reads per sample per metabarcode.

Files

16S_ASV_taxonomy_detailed.txt

Files (201.8 MB)

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md5:1074072aa05cc66209c61d944329e498
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Additional details

Identifiers

Other
Soil biome of Lupinus nipomensis in American Journal of Botany

Related works

Is supplemented by
Publication: Nguyen et al. (Other)

Dates

Available
2024-06-23
ASV tables are available. Associated raw fastq files are pending in NCBI and will be associated soon.

References

  • Coming soon