Dataset Open Access

USP5 Zf-UBD Fluorescence Polarization Displacement Assay Optimization

Mann, Mandeep; Harding, Rachel; Li, Fengling; Schapira, Matthieu; Arrowsmith, Cheryl; Edwards, Aled

Optimization of a fluorescence polarization (FP) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) with an N-terminal FITC-labeled ubiquitin RLRGG peptide.  

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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