COMPARISION OF SERUM AND SALIVA COTININE LEVELS AMONG SMOKERS AND NON SMOKERS BY USING ELISA.
Authors/Creators
- 1. Dept. Of oral pathology, cks theja institute of dental sciences and research, chadalwadanagar, renigunta road, tirupathi-517506
- 2. Dept. Of oral pathology, sreemookambia institute of dental sciences, kulasekharam, kanyakumari-629161
- 3. Director of pathgene health care private limited, tiruchanoor road, tirupathi-517503.
- 4. Dept. Of oral pathology, cks theja institute of dental sciences and research, chadalwadanagar, renigunta road, tirupathi-517506.
- 5. Sri Padmavati mahila viswa vidayalam, padmavathi nagar, tirupathi-517502.
Description
Background: Cotinine, important metabolite of nicotine, is a biomarker for monitoring tobacco exposure from both active smoking and passive smoking. The current study aimed to estimate the cotinine levels and compare the association between serum cotinine and salivary cotinine among smokers and non smokers. Methods: Consecutive consenting individuals (n=50) of 25 smokers (group I) & 25 non smokers (group II) with an age range of 21-75 yrs and with no apparent systemic illness were included in the study and detailed history of smoking was recorded. Serum and unstimulated saliva were collected from each subject in a separate plastic vials and cotinine levels were analyzed using Enzyme-linked immunosorbent assay (ELISA). Results: Among smokers 96% positivity for cotinine was shown in serum & 100% in saliva samples. Among non smokers 40% positivity for cotinine was shown in serum & 80% in saliva samples. When both groups were compared, cotinine positivity was slightly higher in saliva than serum. The results showed cotinine detection was more sensitive in saliva than serum samples. Conclusion: Saliva, being more accessible and noninvasive than serum, it can be used as an alternative diagnostic aid for detection of cotinine mainly for epidemiological surveys and patient education.