Bacterial abundances by flow cytometry - collected from the Southern Ocean in the austral summer of 2016/2017, during the Antarctic Circumnavigation Expedition.

Seawater surface samples (5 m) were collected every 6 hours from the ship’s underway pump. In addition, vertical profiles (6 depths, generally from 5 to 100-150 m) were sampled from CTD casts using a SBE 911 Plus attached to a rosette of 24 12-L PVC Niskin bottles. This dataset presents the abundances of high-DNA containing and low-DNA containing bacteria from seawater samples collected from the ship’s underway pump and CTDs.  Samples were fixed with paraformaldehyde and glutaraldehyde and stored at -80ºC. In the lab, they were thawed, stained with SYBR-Green, and counted in a Cube 8 flow cytometer (SYSMEX PARTEC) based on green fluorewscence. Samples were collected around the Southern Ocean on the R/V Akademik Tryoshnikov in the austral summer of 2016/2017, as part of the Antarctic Circumnavigation Expedition (ACE).