Raw diffraction images of SmgGDS-558 and farnesylated RhoA complex
- 1. Graduate School of Pharmaceutical Sciences, The University of Tokyo
Description
Diffraction images of SmgGDS-558 and farnesylated RhoA complex (PDB code: 5ZHX).
Small and large wedge (1, 5, 180, 360, .. degrees per crystal) datasets were collected from beamlines BL41XU and BL44XU at SPring-8 and BL1A at Photon Factory. PILATUS3 6M (BL41XU), MX300-HS (BL44XU), and EIGER X 4M (BL1A) detectors were used at wavelengths of 0.9 and 1.0 Å.
The crystals belonged to space group P212121 with unit cell parameters a=93.3, b=181.8, c=205.3 Å.
Electron density maps from various combinations of datasets were evaluated through KAMO, and finally data from 20161010_BL44XU/kozo0001 were merged and used for structural refinement at 3.5 Å resolution.
Note that the master.h5 files were modified; see
https://github.com/keitaroyam/yamtbx/blob/master/doc/eiger-en.md
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Additional details
Related works
- Cites
- 10.2210/pdb5zhx/pdb (DOI)
- Is cited by
- 10.1073/pnas.1804740115 (DOI)