Data archive for: Chain forming diatoms use different strategies to avoid diffusion limited N assimilation

Data archive for: “Chain forming diatoms use different strategies to avoid diffusion limited N assimilation” published in Limnology and Oceanography (L&O), https://doi.org/10.1002/lno.12677.

Dataset of single cell assimilation of DIC and NO₃^- by Skeletonema marinoi in the exponential and stationary growth phase captured using secondary ion mass spectrometry (SIMS) and stable isotopic tracers. The data set contains the data used in the study and the outliers excluded from further analysis.

Two strains (Strain 1 and Strain 2) were incubated during either the exponential or stationary growth phase over 24h with ¹⁵N enriched NO₃^- and ¹³C enriched DIC. The cell specific DIC and NO₃^- assimilation was measured using SIMS.

See the main manuscript for a extensive experimental setup and more details.

Each file is uploaded as both a .CSV and .XLSX, so that you can choose which you prefer.

Row description:

Each row represents one Skeletonema marinoi cell.

Column description:

Single.cell: if the cell was a solitary cell (y) or not (n)

End.cell: if the cell was located at the end of a chain (y) or not (n)

Chain_position: a number assigned to identify every cell in a chain starting with 1 at one end of the chain

Chain_length_numeric: the total number of cells in the chain

Chain_length_max_6: the total number of cell in the chain, all numbers larger than 6 are pooled together and labelled >6

Two_chain: if the cell was in a chain consisting of only 2 cells (y) or not (n)

Cell_information: whether the cell was a solitary cell (Single cell), found in a two cell chain (Two cell chain), found at the end of a chain longer than 2 cell (End cell), or in the middle of a chain longer than 3 cells (Middle cell).

Chain: a identifying number assigned to differentiate the different chains

C_fmol_per_cell_day: DIC assimilated in fmol cell^-1 day^-1

N_fmol_per_cell_day: NO₃^- assimilated in fmol cell^-1 day^-1