A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs

EM Quantitation:

Raw data gathered from EM images taken to determine nanodisc population size distribution.

NNB TGN46 analysis:

Data analysis of the Native Nanobleach experiments of TGN46 in native nanodiscs to determine population distribution of oligomeric organizations.

Polymer conditions:

Physiochemical characteristic and extraction conditions for all polymers in the library both commercially available and in-house.

Protein groups polymer screen original file:

Original output of MaxQuant data processing of polymer screen data.

Organelle matching:

Code used for mathcing proteins identified in the proteomics output to organelle or residence for all organellar annotations.

Polymer code:

Code used to process and normalize the MaxQuant output and calulate extraction efficiency across all detected proteins.

MAP Library Details:

Graphic and table explaining chemical details of all polymer used in the screen, both commerically available and in-house synthesized.

NNB TGN46:

Raw scope files for the TIRF microscopy single molecule step photobleaching experiment with TGN46.

Organellar Breakdown Database:

Proteins detected in the polymer screen through proteomics experiments stratified into organelle of residence.

Human Proteome FASTA:

The FASTA file used for proteome searching in processing the proteomics data to build the screening database.

Hand Curated Organellar Proteomes:

Organellar proteomes used for organellar sorting and identification of proteins detected in the screen.

Polymer SEC Superdex75:

Size exculsion chromatography traces for chloroSMA series of polymers. Was used to characterize length and population polydispersity.

Negative Stain Raw:

RAW TEM scope images of purified synaptophysin-vamp2 containing nanodiscs. Populatoin size distribution was determined.

FSEC Polymer CS80:

Fluoresence size exclusion chromatogram for purified synaptophysin-vamp2 containing nanodiscs to ensure population homogeneity and purity.

NMR Raw data:

NMR raw files for characterizing the in-house synthesized Chloro-SMA series and AASTY series.