Published December 14, 2017 | Version v1
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USP5 Zf-UBD Purification & Fluorescence Polarization Displacement Assay Development

  • 1. University of Toronto, Structural Genomics Consortium

Description

USP5 zinc-finger ubiquitin binding domain (Zf-UBD) expression and purification from E. coli, and screening conditions for a fluorescence polarization (FP) displacement assay with a FITC-labelled ubiquitin peptide. 

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20171212_FP_zenodo.pdf

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