Teflubenzuron effects on springtail life history traits explained from impairment of its lipid metabolism

This submission provides xlsx and csv files with data from a comprehensive study aimed at investigating the effects of sublethal concentrations of the insecticide teflubenzuron on the lipid metabolism of Collembola Folsomia candida over different exposure periods.

The dataset files are provided in CSV format with Comma Separated Values:

• Sample_ID_biomass_FolsomiaCandida_RawData.csv
• lipids_positive_mode_raw_data.xlsx
• lipids_negative_mode_raw_data.xlsx
• lipidomics_normalized_data_for_analysis.csv
• lipids_and_exposure_groups_ID.xlsx

Description of the files:

1. The csv file Sample_ID_biomass_FolsomiaCandida_RawData.csv contains the data frame in vertical format with the data for the biomass of Folsomia candida, as well as sample IDs.
2. The xlsx file lipids_positive_mode_raw_data.xlsx contains the annotated lipid features detected in positive mode. The file includes the dataset directly from MS Dial, followed by a sheet with the dataset normalized by sample weight. The last sheet contains the data formatted for further analysis in NORAVA. Lipid IDs were imputed according to Alignment ID.
3. The xlsx file lipids_negative_mode_raw_data.xlsx contains the annotated lipid features detected in negative mode. The file includes the dataset directly from MS Dial, followed by a sheet with the dataset normalized by sample weight. The third sheet contains the data formatted for further analysis in NORAVA. New Lipid IDs for NORAVA were modified and imputed according to Alignment ID and listed on the last sheet.
4. The csv file lipidomics_normalized_data_for_analysis.csv contains the dataset normalized and filtered by QC responses, and further normalized by Eigen MS and log-transformed through NORAVA software. The dataset contains lipids detected from both positive and negative modes and was used for statistical analysis.
5. The xlsx file lipids_and_exposure_groups_ID.xlsx contains two sheets. The first sheet has the IDs for lipid features' names and their IDs used during data analysis. The second sheet contains the exposure group IDs used for NORAVA and information on exposure concentration and exposure time.

Variables in the files:

File 1:

• sample: unique sample ID
• days: day of sampling
• dose: dose of teflubenzuron (insecticide) in soil (mg a.s./kg soil)
• solvent: solvent used in the soil (acetone or water)
• total.biomass (mg): total biomass in mg of the pool of Folsomia candida animals in each sample

Files 2 and 3:

• sample: unique sample ID
• alignment ID: ID of the lipid feature assigned by MS Dial
• metabolite name: name of annotated lipid features
• Values of detected metabolites expressed as peak area

File 4:

• sample: unique sample ID
• label: exposure group ID (see metadata of file 5)
• alignment ID: ID of lipid feature assigned for normalization in NORAVA (see metadata of file 5)

File 5:

Lipid Features ID:

• Alignment ID: ID of lipid feature assigned for normalization in NORAVA
• Lipid_name: full name of annotated lipid features

Exposure Groups:

• label NORAVA: unique exposure group ID
• label_condition: full name of exposure group ID (exposure condition (mg a.s./kg soil) and exposure time)

This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 859891.

This publication reflects only the authors' view, and the European Commission is not responsible for any use that may be made of the information it contains.