Data: Stability and biological response of PEGylated gold nanoparticles
Authors/Creators
- 1. Department of Solid State Engineering, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic
- 2. Institute of Pathological Physiology, 1st Faculty of Medicine, Charles University, 128 53 Prague, Czech Republic
- 3. Faculty of Health Studies, Technical University of Liberec, Liberec, Czech Republic
- 4. Department of Physical Chemistry, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic
- 5. CENAB, Faculty of Science, J. E. Purkyne University, 400 96 Usti nad Labem, Czech Republic
- 6. Institute of Organic Chemistry and Biochemistry of the Czech Academy of Science, 166 10 Prague, Czech Republic
Description
This dataset is focused on thermal stability of PEGylated Au NPs at 4 and 37 °C and after sterilization in autoclave.
Methods
Sputtering of Au into liquid media was performed on Sputter Coater SCD 050 (BalTec AG, CH) apparatus. The sputtering process was carried out at room temperature (RT ~ 25 °C) under following conditions: working gas (Ar, purity 99.999 %, Siad a. s., CZ) pressure of 10 Pa, current of 30 mA, electrode distance of 5 cm. Au was sputtered into 2 ml of capturing media for 900 s. Then the mixture was diluted in distilled water in volume ratio of 1:9 (PEG:H2O). For the study of stability, the Au NP dispersions were thermally stressed in closed vial on magnetic stirrer with heating - Heidolph MR Hei-Tec (Heidolph Instruments GmbH & Co. KG, DE) at 37 or 100 °C or in fridge at 4 °C for 5 h. Au NPs stability was also examined after sterilization in 3870 M Large Capacity Manual Autoclave (Tuttnauer Corp., NL) at temperature of 121 °C for 23 min.
Notes
The authors acknowledge the assistance provided by the Advanced Multiscale Materials for Key Enabling Technologies project, supported by the Ministry of Education, Youth, and Sports of the Czech Republic under the project OP JAK_Amulet No. CZ.02.01.01/00/22_008/0004558 .
Co-funded by the European Union (AR, ZK).
Experiments with SARS-CoV-2 were supported by VES19IZRAEL INTER-EXCELLENCE, subprogram INTER-ACTION LTAIZ19017 from MEYS CR (JW).
Special thanks to Blanka Bilkova for her expertise in cell culture.
Files
Subtraction Result-AuNP-PEG-NH2_256-4_av2.CSV
Files
(430.4 kB)
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Additional details
Related works
- Is published in
- Journal article: 10.1016/j.heliyon.2024.e30601 (DOI)