Published May 2, 2024 | Version v1
Dataset Open

Raw ONT pod5 data: Barcoded D2O Aminobacter P9b DNA

Authors/Creators

  • 1. ROR icon Friedrich Schiller University Jena
  • 2. Friedrich-Schiller-Universität Jena

Description

Aminobacter P9b samples were incubated on a 100% D2O, 30% D2O and 0% D2O medium, respectively. Cells were harvested by centrifugation. Pellets were resuspended and DNA extracted. The DNA was barcoded using the ONTs native barcoding kit, prepared with the SQK-LSK109 protocol, and sequenced on a MinION with an r9.4.1 flowcell.

For barcoding, we used the EXP-NBD114 Barcodes 14, 15, and 16:

BC 14: 0% D2O
BC 15: 30% D2O
BC 16: 100% D2O

Files

Files (198.8 GB)

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Additional details

Additional titles

Subtitle
ONT genomic sequencing of Aminobacter P9b grown on 0% D2O
Subtitle
ONT genomic sequencing of Aminobacter P9b grown on 30% D2O
Alternative title
ONT genomic sequencing of Aminobacter P9b grown on 100% D2O

Funding

Deutsche Forschungsgemeinschaft
Germany´s Excellence Strategy EXC 2051 – Project-ID 390713860
Friedrich Schiller University Jena
Collaborative Research Centre AquaDiva Project-ID 218627073 - SFB 1076
Thüringer Aufbaubank
Thüringer Aufbaubank 2021 FGI 0009
Carl Zeiss Foundation
Scientific Breakthroughs in Artificial Intelligence Interactive Inference

References