World Academy of Science, Engineering and Technology
Published August 1, 2015 | Version 10002400
Journal article Open

Chemical Composition of Essential Oil and in vitro Antibacterial and Anticancer Activity of the Hydroalcolic Extract from Coronilla varia

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The aims of study were investigation on chemical composition essential oil and the effect of extract of Coronilla varia on antimicrobial and cytotoxicity activity. The essential oils of Coronilla varia is obtained by hydrodistillation and analyzed by (GC/MS) for determining their chemical composition and identification of their components. Antibacterial activity of plant extract was determined by disc diffusion method and anticancer activity measured by MTT assay. The major components in essential oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and Homoadantaneca Robexylic Acid (3.31%). The extracts from Coronilla varia had interesting activity against Proteus mirabilis in the concentration of 700 μg/disc and did not show any activity against Staphylococus aureus, Bacillus subtillis, Klebsiella pneumonia and Entrobacter cloacae. The positive control, Ampicillin, Chloramphenicol and Cenphalothin had shown zone of inhibition resistant all bacteria. The ethanol extract of Corohilla varia inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum concentration of extract from Coronilla varia inhibition of cell line growth. The MCF7 cancer cell line and Proteus mirabilis were more sensitive to Coronilla varia ethanol extract.

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References

  • Gonzales GF, Valerio LG.2006. Medicinal plants from Peru: a review of plants as potential agents against cancer. Anticancer Agents Med Chem; 6: 429–444.
  • Rafter, J.J., 2002. Scientific basis of biomarkers and benefits of functional foods for reduction of disease risk: Cancer. Br. J. Nutr., 88: S219-S224.
  • Madhusudan S, Middleton MR. 2005 The immerging role of DNA repair proteins as predictive, prognostic, and therapeutics in cancer. Cancer Treat;31:603-617.
  • Mignogna MD, Fedele S, Lo Russo L. 2004 .The World cancer report and the burden of oral cancer. Eur J Cancer Prev;13:139–142.
  • Cowan MM (1999).Plant products as antimicrobial gents. Clin.Microb., 22:564-582.
  • Reische DW, Lillard DA, Eitenmiller RR1998. Antioxidants in food lipids. In: Ahoh CC, Min DB (Eds.), Chemistry, Nutrition and Biotechnology. Marcel Dekker, New York, pp. 423–448.
  • Sokmen A, Jones BM, Erturk M,1999.Antimicrobial activity of extracts from the cell cultures of some Turkish medicinal plants Phytotherapy Research,13, ( 4)pages 355–357
  • Oka Y, Elisseeva OA, Tsuboi A, Ogawa H, Tamaki H, Li H, Oji Y, Kim EH, Soma T, Asada M, Ueda K, Maruya E, Saji H, Kishimoto T, Udaka K, Sugiyama H. 2000 Human cytotoxic T-lymphocyte responses specific for peptides of the wild-type Wilms' tumor gene (WT1 ) product. Immunogenetics. Feb;51(2):99-107.
  • Launert. E. Edible and Medicinal Plants. Hamlyn 1981 ISBN 0-600- 37216-2 Covers plants in Europe. a drawing of each plant, quite a bit of interesting information. [10] Bauer AW, Kirby MM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45: 493-6. [11] Khajeh M, Yamini Y, Bahramifar N, Sefidkon F, Pirmoradei MR (2005).Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem., 91:639-644. [12] Saikat Dewanjee, Anup maiti, Sanchita Das, Ranabir Sahu Subhash, C. Mandal. 2008. In vitro Antioxidant activity of Diospyros peregrinai fruits, Int J Pharmacol Biol Sci 2(3):135-144. [13] Hashemi. A, Abediankenari S, Ghasemi M, Azadbakht M, Yousefzadeh Y and Dehpour AA. 2011. The effect of Fig Tree Latex (Ficus carica) on Stomach Cancer Line. Iranian Red Crescent Medical Journal,13(4):272- 275