Anti-Aging Effects of Retinol and Alpha Hydroxy Acid on Elastin Fibers of Artificially Photo-Aged Human Dermal Fibroblast Cell Lines
Authors/Creators
Description
Skin aging is a slow multifactorial process influenced
by both internal as well as external factors. Ultra-violet radiations
(UV), diet, smoking and personal habits are the most common
environmental factors that affect skin aging. Fat contents and fibrous
proteins as collagen and elastin are core internal structural
components. The direct influence of UV on elastin integrity and
health is central on aging of skin especially by time. The deposition
of abnormal elastic material is a major marker in a photo-aged skin.
Searching for compounds that may protect against cutaneous photodamage
is exceedingly valued. Retinoids and alpha hydroxy acids
have been endorsed by some researchers as possible candidates for
protecting and or repairing the effect of UV damaged skin. For
consolidating a better system of anti- and protective effects of such
anti-aging agents, we evaluated the combinatory effects of various
dosages of lactic acid and retinol on the dermal fibroblast’s elastin
levels exposed to UV. The UV exposed cells showed significant
reduction in the elastin levels. A combination of drugs with a higher
concentration of lactic acid (30 -35 mM) and a lower concentration of
retinol (10-15mg/mL) showed to work better in maintaining elastin
concentration in UV exposed cells. We assume this preservation
could be the result of increased tropo-elastin gene expression
stimulated by retinol whereas lactic acid probably repaired the UV
irradiated damage by enhancing the amount and integrity of the
elastin fibers.
Files
10000933.pdf
Additional details
References
- S. Ronken, "The effect of ascorbic acid and retinoic acid on elastin and collagen gene expression in monolayers" Identity number: 0527700 BMTE. Accessed from http://www.mate.tue.nl/mate/pdfs/8073.pdf, 2007.
- A. Hayashi, T. Suzuki, and S. Tajima, "Modulations of elastin expression and cell proliferation by retinoids in cultured vascular smooth muscle cells," J. Biochem., vol. 117, pp. 132–136G. Jan 1995.
- J. William, B. Timothy, "Diseases of the Skin: clinical Dermatology", Saunders Elsevier. ISBN 0-7216-2921-0, 2006.
- L. D. Muiznieks, A. S. Weiss and F.W. Keeley, "Structural disorder and dynamics of elastin", Biochem Cell Biol 88 (2): 239–50, 2010.
- J. Uitto, "The role of elastin and collagen in cutaneous aging: Intrinsic aging versus photoexposure", J Drugs Dermatol. 2008 Feb; 7 (2 Suppl): s12-6, 2008.
- E. F Bernstein, Y. Q Chen, K. Tamai, K. Shepley, K. S. Resnik, H. Zhang, R. Tuan, A. Mauviel, J. Uitto, "Enhanced elastin and fibrillin gene expression in chronically photodamaged skin", J Invest Dermatol 103:182-186, 1994.
- G. A. Di Lullo, S. M. Sweeney, J. Körkkö, L. Ala-Kokko, J. D. San Antonio, "Mapping the Ligand-binding Sites and Disease-associated Mutations on the Most Abundant Protein in the Human, Type I Collagen", J. Biol. Chem. 277 (6): 4223–4231, 2002.
- M. Ramos, E. Silva, D. M. Hexsel, M. S. Rutowitsch, "Hydroxy acids and retinoids in cosmetics, "ClinDermatol; 19:460–6, 2001.
- E. J. Van Scott, R. J. Yu, "Alpha hydroxy acids: Procedures for use in clinical practice", 43:222–8, 1989. [10] C. M. Ditre, T.D Griffin, G. F. Murphy, E. J. Van Scott, "Improvement of photodamaged skin with alpha-hydroxy acid (AHA): A clinical, histological, and ultra-structural study", Dermatology Congress. Vienna, Austria. May 18–21, 1993:175, 2000. [11] X. Wang. "A theory for the mechanism of action of the alpha-hydroxy acids applied to the skin", Medical Hypotheses; 53:120 –2, 1999. [12] M. N. O'Donoghue. "Update on acne therapy". Dermatol Nurs; 11:205– 8, 1999.