World Academy of Science, Engineering and Technology
Published August 1, 2014 | Version 9999239
Journal article Open

In situ Biodegradation of Endosulfan, Imidacloprid, and Carbendazim Using Indigenous Bacterial Cultures of Agriculture Fields of Uttarakhand, India

Description

In the present study, presence of endosulfan, imidacloprid, carbendazim, in the soil /vegetables/cereals and water samples was observed in agriculture fields of Uttarakhand. In view of biodegradation of these pesticides, 9 bacterial isolates were recovered from the soil samples of the fields which tolerated endosulfan, imidacloprid, carbendazim from 100 to 200 µg/ml. Three bacterial consortia used for in vitro bioremediation experiments were consisted of 3 bacterial isolates for carbendazim, imidacloprid and endosulfan, respectively. Maximum degradation (87 and 83%) of α and β endosulfan respectively was observed in soil slurry by consortium. Degradation of Imidacloprid and carbendazim under similar conditions was 88.4 and 77.5% respectively. FT-IR analysis of biodegraded samples of pesticides in liquid media showed stretching of various bonds. GC-MS of biodegraded endosulfan sample in soil slurry showed the presence of nontoxic intermediates. A pot trial with Bacterial treatments lowered down the uptake of pesticides in onion plants.

Files

9999239.pdf

Files (223.9 kB)

Name Size
md5:4fd60c739722ae72da6a6f1c207644ea
223.9 kB Preview Download

Additional details

References

  • G.T. Miller, Sustaining the Earth, 6th edition. Thompson Learning, Inc. Pacific Grove, California. Chapter 9, 2004. pp. 211-216.
  • S.W. Kullman, and F. Matsumura, "Metabolic pathway utilized by Phanerochaete chrysosporium for degradation of the cyclodiene pesticide endosulfan", Appl. Environ. Microbiol. Vol. 62, 1996, pp. 880-600.
  • N. Sinha; R. Narayan,. and D.K. Saxena, "Effect of endosulfan on testis of growing rats",. Bull. Environ. Contam. Toxicol. 1997vol 58 pp.79-86.
  • H. Goebel, S. Gorbach, W. Knauf, R.H Rimpau,. and H. Huttenbach, "Properties, effects, residues and analytics of the insecticide endosulfan", Residue Reviews 1982 vol 83:pp.1-122.
  • N.S. Singh, and D.K. Singh, "Biodegradation of endosulfan and endosulfan sulfate by Achromobacter xylosoxidans strain C8B in broth medium", Biodegradation. 2011. Vol 22, pp.845–857.
  • H. Saiyed, A. Dewan, V. Bhatnagar, U. Shenoy, R. Shenoy, H. Rajmohan, K. Patel, R. Kashyap, P. Kulkarni, B. Rajan, and B. Lakkad, "Effect of endosulfan on male reproductive development", Environ Health Perspect. 2003. Vol. 111, pp.1958–1962.
  • N. Awasthi, R. Ahuja, and A. Kumar, "Factors influencing the degradation of soil applied endosulfan isomers", Soil. Biol. Biochem. 2000 vol. 32, pp.1697–1705.
  • S. Hussain, M. Arshad, M. Saleem, and Z.A. Zahir, "Screening of soil fungi for in vitro degradation of endosulfan.",World J Microbiol Biotechnol", 2007 vol. 23,pp.939–945.
  • J.A Gervais, B. Luukinen, K. Buhl, and D. Stone, "Imidacloprid Technical Fact Sheet",NationalPesticide Information Center. 2010. [10] D.A. Muccio, P. Fidente, D. A. Barbini, R. Dommarco, S. Seccia, and P. Morrica, "Application of soild-phase extraction and liquid chromatography-mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables", J. Chromatogr. A. 2006 vol. 1108, pp.1-16. [11] J. Rouchaud, F. Gustin, and A. Wauters, "Imidacloprid insecticide soil metabolism in sugar beet field crops", Bull. Environ. Contam. Toxi-col. 1996,vol. 56, pp.29-36. [12] J.C. Anhalt, T.B Moorman, and W.C. Koskinen, "Biodegradation of imidacloprid by an isolated soil microorganism", J. Environ. Sci. Health Part B. 2007, vol. 42, pp.509–514. [13] J. Xu, X. Gu, B. Shen, Z.Wang, K. Wang, and S. Li,. "Isolation and Characterization of a Carbendazim-Degrading Rhodococcus sp. djl-6", Current Microbiolog, 2006, vol. 53,pp. 72-76. [14] M. Anastassiades, S.J. Lehotay, D. Stajnbaher, and F.J. Schenck, "Fast and easy multiresidue method employing extraction/partitioning and dispersive solid phase extrac¬tion for the determination of pesticide residues in pro¬duce", J AOAC Int. 2003, vol. 86, pp.412-431. [15] S.P. Kale, N.B.K. Munhy, and K. Raghu, "Effect of carbofuran, carbaryl and their metabolites on the growth of Rhizobium sp. and Azotobacter chroococcum",Bull. Environ. Contam. Toxicol. 1989, vol 42, pp.769-772. [16] M. Bazzicalupo, and R. Fani, "The use of RAPD for generating specific DNA probes for microorganisms",Clap J. P., Ed. Methods in Molecular Biology. Species Diagnostic Protocols: PCR and Other Nucleic Acid Methods.Humana Press Inc. Totowa N.J: 1995, pp. 155-175. [17] R. Kataoka, K. Takagi, and F. Sakakibara, "A new endosulfan-degrading fungus, Mortierella species, isolated from a soil contaminated with organochlorine pesticides", J. Pestic. Sci., 2010, vol 35, pp.326–332. [18] X.Wang, D.Wang, X. Qin, and X. Xu, "Residues of organochlorine pesticides in surface soils from college school yards in Beijing, China", J Environ Sci, 2008, vol, 20, pp.1090-1096. [19] S. Sunitha, K. V. Murthy, and R. Mahmood, "Degradation of Endosulfan by Mixed Bacterial Cultures Enriched from Endosulfan Contaminated Soils of Southern India", Int. J. Bioscience, Biochem. Bioinfo, 2012, vol. 2, pp.31-35. [20] X. Zhang, T. Jia, X. M. Cheng, and Y. Zhao, "Isolation and characterization of a new carbendazim-degrading Ralstonia sp. Strain",World J. of Microbiol. & Biotechnol,2005,vol. 21, pp.265- 269. [21] H.K. Manonmani, D.H. Chandrashekaraiah, N. Shridharreddy, C.D. Elcey, and A.A.M. Kunhi, "Isolation and acclimatization of microbial consortium for improved aerobic degradation of γ-hexachlorocyclohexane", J. Agric. Food. Chem,2000, vol .48, pp.4341–4351. [22] M. Pandey, S.Phartyal, A. Srivastava, and A.Sharma, "Degradation of -Hexachlorocyclohexane by Gordonia species Isolated from Contaminated Field Soil", Pesticide Research Journal 2012. vol. (24) 2,pp.199-204. [23] A.A Shetti,. and B.B. Kaliwal, "Biodegradation of imidacloprid by soil isolate Brevundimonas sp. Mj15", International Journal of Current Research, 2012, vol. 4 ,pp.100-106. [24] S. Prajapati, S. Phartyal, G Negi, and A.Sharma, "Degradation of Chlorpyrifos by Indigenous Soil Bacteria", Pesticide Research Journal, 2012, vol .(24) 2, pp.173-176. [25] B.C. Okeke, T. Siddique, M.C. Arbestain, and W.T. Frankenberger, "Biodegradation of γ-hexachlorocyclohexane (Lindane) and α-hexachlorocyclohexane in water and a soil slurry by a Pandoraea species", J. Agr. Food Chem, 2002,vol. 50, pp.2548–2555. [26] A. Nawab, A. Aleem, and A. Malik, "Determination of organo-chlorine pesticides in agricultural soil with special reference to γ-HCH degradation by Pseudomonas strains.",Bioresource Technology,2003, vol .88, pp.41–46. [27] L Tian, and F.Chen, "Characterization of a carbendazim-degrading Trichoderma sp. T2-2 and its application in bioremediation", Acta Microbiologica, 2009,vol. 49, pp.925-930. [28] M. Kumar, and L. Philip, "Bioremediation of endosulfan contaminated soil and water – optimization of operating conditions in laboratory scale reactors", J Hazard Mater, 2006, vol. 136, pp.354–364. [29] M. Kumar, C.V. Lakshmi, and S. Khanna, "Biodegradation and bioremediation of endosulfan contaminated soil", Bioresource Technology, 2008,vol. 99,pp. 3116–3122. [30] S. Kumar, "Bioremediation of endosulfan by bacteria isolated from the agricultural fields", Int. J. of Pharma and Bio Sciences, 2011,vol.3,pp.367-374. [31] R. H. M. Murthy, M. S. Thakur, and H. K. Manonmani, "Degradation of Technical Grade Hexachlorocyclohexane In Soil Slurry by a Defined Microbial Consortium", Int. J. Environ. Res, 2010,vol. 4, pp.471-478. [32] R.L.Irvine, J.P.Earley, G.J Kehrberger, and B.T. Delaney, "Bioremediation of soils contaminated with bis- (2-ethylhexyl) phthalate (BEHP) in a soil slurry-sequencing batch reactor", Environ. Prog,1993, vol.2,pp.39-44. [33] N.Vijayan, R. Babu, R.Gopalakrishnan, P. Ramasamy, and W.T.A. Harrison, "Growth and characterization of benzimidazole single crystals: a nonlinear optical material",J. Cyrstal Growth, 2004,vol .262, pp.490-498. [34] A.S. Nair, R.T Tom, and T. Pradeep, "Detection and extraction of endosulfan by metal nanoparticles", J. Environ. Monit,2003, vol. 5,pp.363–365. [35] K.M.Weir, T.D.Sutherland, I.Horne, R.J. Russell, and J.G. Oakeshott, "A single monooxygenase, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.", Appl Environ Microbiol,2006, vol. 72,pp.3524–3530. [36] K.K. Dubey, and M.H. Fulekar, "Effect of pesticides on the seed germination of Cenchrus setigerus and Pennisetum pedicellatum as monocropping and co-cropping system: implications for rhizospheric bioremediation",Rom. Biotech. Letters, 2011,vol.16, pp.5908–5918. [37] C.S.Benimeli, M.S.Fuentes, C.M. Abate, and M.J. Amoroso, "Bioremediation of lindane contaminated soil by Streptomyces sp. M7 and its effect on Zea mays growth", Int. Biodet. & Biodeg, 2008, vol. 61,pp. 233-239. [38] S. Phartyal, "Characterization of Lindane degrading Bacteria from tarai region of uttarakhand and their potential assessment in bioremediation", 2012,Ph.D Thesis, GBPUA&T Pantnagar, India.