World Academy of Science, Engineering and Technology
Published September 24, 2013 | Version 16607
Journal article Open

Wound Healing Effect of Ocimum sanctum Leaves Extract in Diabetic Rats

Description

Delayed wound healing in diabetes is primarily
associated with hyperglycemia, over-expression of inflammatory
marker, oxidative stress and delayed collagen synthesis. This
unmanaged wound is producing high economic burden on the
society. Thus research is required to develop new and effective
treatment strategies to deal with this emerging issue. Our present
study incorporates the evaluation of wound healing effects of 50%
ethanol extract of Ocimum sanctum (OSE) in streptozotocin
(45mg/kg)-induced diabetic rats with concurrent wound ulcer. The
animals showing diabetes (Blood glucose level >140 and <250
mg/dL) will be selected for wound healing study using standard dead
space wound model. Wounds were created by implanting two
polypropylene tubes (0.5 x 2.5 cm2 each), one on either side in the
lumbar region on the dorsal surface of each rat. On the 10th postwounding
day, the animals were sacrificed and granulation tissue
formed on the implanted tubes was carefully dissected out and study
the status of antioxidants (Superoxide dismutase, SOD and
Glutathione, GSH) free radicals (Lipid peroxidation, LPO and nitric
oxide, NO) acute inflammatory marker (myeloperoxidase, MPO)
connective tissue determinants, hydroxyproline, hexosamine and
hexuronic acid, which play a major role in wound healing and
diabetes. Besides the anti-diabetic parameters (estimation of serum
blood glucose, triglycerides and total cholesterol), the above
parameters for wound healing were studied both in normal, untreated
and OSE treated diabetic rats. The effects of extract on above
parameters will be compared with known standard antioxidant
(Vitamin E) and anti-diabetic (Glybenclamide) drugs. OSE 400
mg/kg substantiated by significantly decreased serum blood glucose,
triglycerides and total cholesterol. OSE also decrease granulation
tissue free radicals (LPO, 58.1% and NO, 52.7%) and
myeloperoxidase (MPO, 63.3%), and enhanced antioxidants (GSH,
116.4% and SOD, 201.1%)

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References

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