scRNA-seq data of: A novel in vitro tubular model to recapitulate features of distal airways: the bronchioid

We provide a .Rds file of an annotated Seurat Object of scRNA-seq data of two bronchioid models derived from distinct donors after 21days of culture using 10x genomics 3' v3 chemistry. Raw data was processed using CellRanger v7.1.0. Cells were filtered based on detected UMIs (>2000) and fraction of mitochondrial counts (<10%).
Metadata annotations contain:
- Patient -> patient information for every cell (patient1 or patient2)
- nCount_RNA -> UMI counts per cell
- nFeature_RNA -> genes detected per cell
- percent.mt -> mitochondrial count fraction per cell
- seurat_clusters -> unsupervised clustering results using Louvain algorithm with resolution = 0.5
- Manual.Annotation -> Cell types annotated based on marker gene expression
- Celltypist.prediction -> Cell types predicted with CellTypist Python package
- Celltypist.prediction.ari -> Cell types predicted with CellTypist Python package, with harmonized names for comparison with manual annotation