Published September 2, 2025
| Version v2
Dataset
Open
Cryo-4D-STEM datasets on cells and cellular organelles for demonstrating a dose-Efficient cryo-EM technique: tilt-Corrected Scanning Transmission Electron Microscopy
Authors/Creators
- 1. Chan Zuckerberg Institute for Advanced Biological Imaging
Description
This upload contains three 4D-STEM datasets in .raw format for demonstrating a dose-efficient cryo-EM technique for thick samples: tilt-corrected Scanning Transmission Electron Microscopy (tcBF-STEM). The dataset dimension is 128130256*256. Data were acquired on vitrified intact E.coli cells and isolated human cell organelles. This upload also contains the EFTEM images in .mrc acqired in the same ROI as the 4D-STEM dataset.
It also contains analysis of the manuscript's Fig 3 and Ext. data fig 8.
Technical info
Metadata:
4DSTEM datasets
- scan step size: 2.8nm
- semi-convergence angle (nominal): 7 mrad
- dose: 14e-/Å2
Files
ED_fig8_plotlineprofiles.ipynb
Files
(334.2 kB)
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