The VSMC RNA-seq datasets for "Strategies for Arterial Grafts Optimization at Single Cell Level"

This dataset collection contains the human vascular smooth muscle cell (VSMC) bulk RNA-seq data generated in the study Strategies for Arterial Grafts Optimization at Single Cell Level.

Primary human VSMCs (CP-H167, Pricella) were cultured in DM/F12 medium supplemented with FBS, EGF, bFGF, insulin, and penicillin (CM-P167, Pricella). The media was replaced per 48 hours. VSMCs were treated with 100ng/ml MIF (HY-P7387, MCE) for 24 hours in the absence or presence of 50 µMol ISO1 (HY-16692, MCE). The VSMCs with the passage number 3 to 5 were used in the study.

Total RNA was extracted from cultured VSMC under different treatments using RNeasy Mini Kit (74104, Qiagen). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. The library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated. The index of the reference genome was built using Hisat2 v2.0.5. and paired-end clean reads were aligned to the reference genome using Hisat2 v2.0.5.

The sample info for the BAM files and their index files are listed as follows: