Published February 27, 2024
| Version v1
Journal article
Open
Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China
Authors/Creators
- 1. Beijing Forestry University, Beijing, China
- 2. National Centre for Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore|University of British Columbia, Vancouver, Canada
Description
Chinese yew, Taxus chinensis var. mairei is an endangered shrub native to south-eastern China and is widely known for its medicinal value. The increased cultivation of Chinese yew has increased the incidence of various fungal diseases. In this study, Pestalotioid fungi associated with needle spot of Chinese yew were isolated from Guangxi Province. Based on morphological examinations and multi-locus (ITS, tub2, tef-1α) phylogenies, these isolates were identified to five species, including two new species, Pestalotiopsis taxicola and P. multicolor, two potential novel Neopestalotiopsis species, Neopestalotiopsis sp. 3 and Neopestalotiopsis sp. 4, with a known Pestalotiopsis species (Pestalotiopsis trachycarpicola), firstly recorded from Chinese yew. These two new Pestalotiopsis species were morphologically and phylogenetically distinct from the extant Pestalotioid species in Chinese yew. Pathogenicity and culture characteristic tests of these five Pestalotioid species were also performed in this study. The pathogenicity test results revealed that Neopestalotiopsis sp. 3 can cause diseases in Chinese yew needles. These results have indicated that the diversity of Pestalotioid species associated with Chinese yew was greater than previously determined and provided helpful information for Chinese yew disease diagnosis and management.
Files
MC_article_113696.pdf
Files
(8.0 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:0be1e89ab84edcfd3a37da5769a11dbb
|
8.0 MB | Preview Download |
System files
(176.0 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:27b0aa431425055abb17941946ac4861
|
176.0 kB | Download |
Linked records
Additional details
References
- Alves A, Crous PW, Correia A, Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1–13. https://doi.org/10.1002/yea.1554
- Araujo L, Ferreira Pinto FAM, de Andrade CCL, Mituti T, Falkenbach BR, Gomes LB, Duarte V (2023) Pestalotiopsis trachycarpicola causes leaf spot disease on blueberry in Santa Catarina, Brazil. Australasian Plant Disease Notes, Australasian Plant Pathology Society 18(1): 14. https://doi.org/10.1007/s13314-023-00500-7
- Chaiwan N, Wanasinghe DN, Mapook A, Jayawardena RS, Norphanphoun C, Hyde KD (2020) Novel species of Pestalotiopsis fungi on Dracaena from Thailand. Mycology 11(04): 306–315. https://doi.org/10.1080/21501203.2020.1801873
- Chen Y, Wei G, Chen W (2002) New species of Pestalotiopsis. Mycosystema 21(03): 316–323.
- Chen Y, Wan Y, Zeng L, Meng Q, Yuan L, Tong H (2021) Characterization of Pestalotiopsis chamaeropis causing gray blight disease on tea leaves (Camellia sinensis) in Chongqing, China. Canadian Journal of Plant Pathology 43(3): 413–420. https://doi.org/10.1080/07060661.2020.1816582
- Ding G, Zheng Z, Liu S, Zhang H, Guo L, Che Y (2009) Photinides A-F, cytotoxic benzofuranone-derived γ-Lactones from the plant endophytic fungus Pestalotiopsis photiniae. Journal of Natural Products 72(5): 942–945. https://doi.org/10.1021/np900084d
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus (San Francisco, Calif. ) 12: 13–15.
- Fan X, Zhu J, Zhou X, Meng X, Lin Z, Guo W (2006) The identification and biological characteristics of the anthracnose pathogen of Southern Chinese yew. Journal-Fujian College Forestry 26(2): 117–122.
- Fan J, Qiu HB, Long HJ, Zhao W, Xiang XL, Hu AL (2021) First report of leaf spot on sorghum caused by Pestalotiopsis trachycarpicola in China. Journal of Plant Pathology 103(3): 1043–1044. https://doi.org/10.1007/s42161-021-00853-x
- Freitas EFS, Silva MD, Barros MVP, Kasuya MCM (2019) Neopestalotiopsis hadrolaeliae sp. nov., a new endophytic species from the roots of the endangered orchid Hadrolaelia jongheana in Brazil. Phytotaxa 416(3): 211–220. https://doi.org/10.11646/phytotaxa.416.3.2
- Gao ZW (2006) Research on Taxus chinensis var. mairei. China Forestry Publishing House, China, 143 pp.
- Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61(4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
- Ismail AM, Cirvilleri G, Polizzi G (2013) Characterisation and pathogenicity of Pestalotiopsis uvicola and Pestalotiopsis clavispora causing grey leaf spot of mango (Mangifera indica L.) in Italy. European Journal of Plant Pathology 135(4): 619–625. https://doi.org/10.1007/s10658-012-0117-z
- Jeon YH, Cheon W (2014) First report of leaf blight of Japanese yew caused by Pestalotiopsis microspora in Korea. Plant Disease 98(05): 691–691. https://doi.org/10.1094/PDIS-08-13-0821-PDN
- Jiang N, Fan X, Tian C (2021) Identification and characterization of leaf-Inhabiting fungi from Castanea plantations in China. Journal of Fungi 7(1): 64. https://doi.org/10.3390/jof7010064
- Jiang N, Voglmayr H, Xue H, Piao CG, Li Y (2022) Morphology and phylogeny of Pestalotiopsis (Sporocadaceae, Amphisphaeriales) from Fagaceae leaves in China. Microbiology Spectrum 10(6): e03272–e22. https://doi.org/10.1128/spectrum.03272-22
- Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev/mst010
- Keith RC, Keith LM, Hernandez-Guzman G, Uppalapati SR, Bender CL (2003) Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host and non-host plants. Microbiology (Reading, England) 149(5): 1127–1138. https://doi.org/10.1099/mic.0.26109-0
- Khalilabad AA, Fotouhifar KB (2022) Morphological and molecular characterization of a novel Pestalotiopsis trachycarpicola, causing Garden Croton leaf spot in Iran. Mycologia Iranica 9(1): 59–66.
- Kumaran RS, Kim HJ, Hur BK (2010) Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering 110(5): 541–546. https://doi.org/10.1016/j.jbiosc.2010.06.007
- Lan CZ, Lin X, Dai YL, Gan L, Liu XF, Ruan HC, Yang XJ (2023) First report of leaf blight on Panax notoginseng caused by Pestalotiopsis trachycarpicola in China. Journal of Plant Pathology 105(1): 321–321. https://doi.org/10.1007/s42161-022-01236-6
- Li Y (2017) Identification of the leaf spot pathogen on Chinese yew and screening of fungicides to Pestalotiopsis microspora. PhD Thesis, Fujian Agriculture and Forestry University, Fujian, China.
- Li C, Huo C, Zhang M, Shi Q (2008) Chemistry of Chinese yew, Taxus chinensis var. mairei. Biochemical Systematics and Ecology 36(4): 266–282. https://doi.org/10.1016/j.bse.2007.08.002
- Li K, Zhang G, Zhang Y, Griffith MP (2021a) A noteworthy case of rewilding Chinese yew from a garden population in eastern China. PeerJ 9: e12341. https://doi.org/10.7717/peerj.12341
- Li L, Yang Q, Li H (2021b) Morphology, phylogeny, and pathogenicity of Pestalotioid species on Camellia oleifera in China. Journal of Fungi (Basel, Switzerland) 7(12): 1080. https://doi.org/10.3390/jof7121080
- Liu AR, Chen SC, Wu SY, Xu T, Guo LD, Jeewon R, Wei JG (2010) Cultural studies coupled with DNA based sequence analyses and its implication on pigmentation as a phylogenetic marker in Pestalotiopsis taxonomy. Molecular Phylogenetics and Evolution 57(2): 528–535. https://doi.org/10.1016/j.ympev.2010.07.017
- Liu L, Wang Z, Huang L, Wang T, Su Y (2019) Chloroplast population genetics reveals low levels of genetic variation and conformation to the central-marginal hypothesis in Taxus wallichiana var. mairei, an endangered conifer endemic to China. Ecology and Evolution 9(20): 11944–11956. https://doi.org/10.1002/ece3.5703
- Ma XY, Maharachchikumbura SSN, Chen BW, Hyde KD, Mckenzie EHC, Chomnunti P, Kang JC (2019) Endophytic pestalotiod taxa in Dendrobium orchids. Phytotaxa 419(3): 268–286. https://doi.org/10.11646/phytotaxa.419.3.2
- Maharachchikumbura SSN, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EHC, Bahkali A, Hyde KD (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterizatio of 14 new species. Fungal Diversity 56(1): 95–129. https://doi.org/10.1007/s13225-012-0198-1
- Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW (2014) Pestalotiopsis revisited. Studies in Mycology 79(1): 121–186. https://doi.org/10.1016/j.simyco.2014.09.005
- Moslemi A, Taylor PWJ (2015) Pestalotiopsis chamaeropis causing leaf spot disease of round leaf mint-bush (Prostanthera rotundifolia) in Australia. Australasian Plant Disease Notes, Australasian Plant Pathology Society 10(1): 1–5. https://doi.org/10.1007/s13314-015-0179-9
- Nozawa S, Seto Y, Watanabe K (2019) First report of leaf blight caused by Pestalotiopsis chamaeropis and Neopestalotiopsis sp. in Japanese andromeda. Journal of General Plant Pathology 85(6): 449–452. https://doi.org/10.1007/s10327-019-00868-4
- Pyo SH, Park HB, Song BK, Han BH, Kim JH (2004) A large-scale purification of paclitaxel from cell cultures of Taxus chinensis. Process Biochemistry (Barking, London, England) 39(12): 1985–1991. https://doi.org/10.1016/j.procbio.2003.09.028
- Qi XL, He J, Ju Y, Huang L (2022) First report of leaf spot on Elaeagnus pungens caused by Neopestalotiopsis clavispora in China. Plant Disease 107(7): 2251. https://doi.org/10.1094/PDIS-10-22-2457-PDN
- Qian TM, Rong L, Zhu TH (2015) ldentification of the pathogen isolated from root rot of Taxus chinensis var. mairei and screening of its antagonistic Bacillus. Plant Protection 41(06): 60–66.
- Rambaut A (2016) FigTree v1.4.3. Institute of Evolutionary Biology, University of Edinburgh, UK. http://tree.bio.ed.ac.uk/software/figtree
- Ran SF, Maharachchikumbura SSN, Ren YL, Liu H, Chen KR, Wang YX, Wang Y (2017) Two new records in Pestalotiopsidaceae associated with Orchidaceae disease in Guangxi Province, China. Mycosphere 8(01): 121–130. https://doi.org/10.5943/mycosphere/8/1/11
- Reddy MS, Murali TS, Suryanarayanan TS, Rajulu MG, Thirunavukkarasu N (2016) Pestalotiopsis species occur as generalist endophytes in trees of Western Ghats forests of southern India. Fungal Ecology 24: 70–75. https://doi.org/10.1016/j.funeco.2016.09.002
- Ru WM (2006) Study on the ecology of endangering plant Taxus chinensis var. mairei. PhD Thesis, Shanxi University, Shanxi, China.
- Rubini MR, Silva-Ribeiro RT, Pomella AW, Maki CS, Araújo WL, Dos Santos DR, Azevedo JL (2005) Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches' Broom Disease. International Journal of Biological Sciences 1: 24–33. https://doi.org/10.7150/ijbs.1.24
- Shu J, Yu Z, Sun W, Zhao J, Li Q, Tang L, Guo T, Huang S, Mo J, Hsiang T, Luo S (2020) Identification and characterization of pestalotioid fungi causing leaf spots on mango in southern China. Plant Disease 104(4): 1207–1213. https://doi.org/10.1094/PDIS-03-19-0438-RE
- Stamatakis A (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Strobel G, Yang X, Sears J, Kramer R, Sidhu RS, Hess WM (1996) Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallichiana. Microbiology (Reading, England) 142(2): 435–440. https://doi.org/10.1099/13500872-142-2-435
- Subban K, Subramani R, Srinivasan VPM, Johnpaul M, Chelliah J (2019) Salicylic acid as an effective elicitor for improved taxol production in endophytic fungus Pestalotiopsis microspora. PLOS ONE 14(2): e0212736. https://doi.org/10.1371/journal.pone.0212736
- Sun YR, Jayawardena RS, Sun JE, Wang Y (2023) Pestalotioid species associated with medicinal plants in southwest China and Thailand. Microbiology Spectrum 11(1): e03987–e22. https://doi.org/10.1128/spectrum.03987-22
- Swofford D (2003) PAUP* - Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts, USA.
- Wang R, Chen S, Zheng B, Liu P, Li B, Weng Q, Chen Q (2019) Occurrence of leaf spot disease caused by Neopestalotiopsis clavispora on Taxus chinensis in China. Forest Pathology 49(5): e12540. https://doi.org/10.1111/efp.12540
- White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR-Protocols: A guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Wu L, Han T, Li W, Jia M, Xue L, Rahman K, Qin L (2013) Geographic and tissue influences on endophytic fungal communities of Taxus chinensis var. mairei in China. Current Microbiology 66(1): 40–48. https://doi.org/10.1007/s00284-012-0235-z
- Xiong Y, Manawasinghe I, Maharachchikumbura SSN, Dong ZY, Xiang MM, Li L (2022) Pestalotioid species associated with palm species from Southern China. Current Research in Environmental & Applied Mycology 12(1): 285–321. https://doi.org/10.5943/cream/12/1/18
- Yang Q, Zeng XY, Yuan J, Zhang Q, He YK, Wang Y (2021) Two new species of Neopestalotiopsis from southern China. Biodiversity Data Journal 9: e70446. https://doi.org/10.3897/BDJ.9.e70446
- Zhang Y, Maharachchikumbura SS, Mckenzie EH, Hyde KD (2012) A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie. Mycologie 33(3): 311–318. https://doi.org/10.7872/crym.v33.iss3.2012.311
- Zhang GF, Li Q, Hou XR (2018) Structural diversity of naturally regenerating Chinese yew (Taxus wallichiana var. mairei) populations in ex situ conservation. Nordic Journal of Botany 36(4): njb–01717. https://doi.org/10.1111/njb.01717
- Zhang Z, Liu R, Liu S, Mu T, Zhang X, Xia J (2022) Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China. MycoKeys 88: 171–192. https://doi.org/10.3897/mycokeys.88.82229