Published February 20, 2024
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Using scanning electron microscopy and molecular data to discover a new species from old herbarium collections: The case of Phlomoides henryi (Lamiaceae, Lamioideae)
Authors/Creators
- 1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China|Inner Mongolia University, Hohhot, China
- 2. Chinese Academy of Sciences, Chengdu Institute of Biology, Chengdu, China
- 3. Royal Botanic Gardens, Kew, United Kingdom
- 4. University of Chinese Academy of Sciences, Beijing, China|Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
- 5. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
Description
Phlomoides is one of the largest genera of Lamiaceae with approximately 150–170 species distributed mainly in Eurasia. In this study, we describe and illustrate a new species, P. henryi, which was previously misidentified as P. bracteosa, from Yunnan Province, southwest China. Molecular phylogenetic analyses revealed that P. henryi is found within a clade in which most species lack basal leaves. In this clade, the new species is morphologically distinct from P. rotata in having an obvious stem and, from the rest, by having transparent to white trichomes inside the upper corolla lip. In addition, micro-features of trichomes on the calyx and leaf epidermis can differentiate the new species from other species grouped in the same clade and a key, based on trichome morphology for these species, is provided. The findings demonstrate that the use of scanning electron microscopy can reveal inconspicuous morphological affinities amongst morphologically similar species and play an important role in the taxonomic study of the genus Phlomoides.
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References
- Astanova SB (1984) Chromosome numbers in the species of the families Alliaceae Asteraceae Caryophyllaceae Ebenaceae Linaceae Oleaceae Lamiaceae from Tadjikistan. Botanicheskii Zhurnal 69(11): 1563–1564.
- Azizian D, Cutler DF (1982) Anatomical, cytological and phytochemical studies on Phlomis L. and Eremostachys Bunge (Labiatae). Botanical Journal of the Linnean Society 85(4): 249–281. https://doi.org/10.1111/j.1095-8339.1982.tb00373.x
- Bebber DP, Carine MA, Wood JRI, Wortley AH, Harris DJ, Prance GT, Davidse G, Paige J, Pennington TD, Robson NKB, Scotland RW (2010) Herbaria are a major frontier for species discovery. Proceedings of the National Academy of Sciences of the United States of America 107(51): 22169–22171. https://doi.org/10.1073/pnas.1011841108
- Bendiksby M, Thorbek L, Scheen A-C, Lindqvist C, Ryding O (2011) An updated phylogeny and classification of Lamiaceae subfamily Lamioideae. Taxon 60(2): 471–484. https://doi.org/10.1002/tax.602015
- Chen YP, Hu GX, Xiang CL (2014) Isodon delavayi (Ocimeae, Nepetoideae, Lamiaceae): A new species from Yunnan Province, Southwest China. Phytotaxa 156(5): 291–297. https://doi.org/10.11646/phytotaxa.156.5.5
- Czerepanov SK (1995) Labiatae. In: Czerepanov SK (Ed.) Vascular Plants of Russia and Adjacent States. Cambridge University Press, Cambridge, 295–305.
- Darriba D, Taboada GL, Doallo R, Posada D (2012) jModel-Test 2: More models, new heuristics and parallel computing. Nature Methods 9(8): e772. https://doi.org/10.1038/nmeth.2109
- Dong HJ, Jamzad Z, Xiang CL (2015) Nepeta wuana (Nepetinae, Nepetoideae, Lamiaceae), a new species from Shanxi, China. Iranian Journal of Botany 21(1): 13–18.
- Doyle JJ, Doyle JD (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19(1): 11–15.
- Fang LQ, Pan YZ, Gong X (2007) A karyomorphological study in the monotypic genus Lamiophlomis and five species in Phlomis (Lamiaceae). Zhiwu Fenlei Xuebao 45(5): 627–632. https://doi.org/10.1360/aps050192
- Gairola S, Naidoo Y, Bhatt A, Nicholas A (2009) An investigation of the foliar trichomes of Tetradenia riparia (Hochst.) Codd (Lamiaceae): An important medicinal plant of Southern Africa. Flora 204(4): 325–330. https://doi.org/10.1016/j.flora.2008.04.002
- Ghaffari SM (2006) New or rare chromosome counts of some angiosperm species from Iran. Iranian Journal of Botany 11(2): 185–192.
- Hsuan SJ (1977) Phlomis. In: Wu CY, Li HW (Eds) Flora Reipublicae Popularis Sinicae (Vol. 65). Science Press, Beijing, 428–478.
- Hu GX, Balangcod TD, Xiang CL (2012) Trichome micromorphology of the Chinese-Himalayan genus Colquhounia (Lamiaceae), with emphasis on taxonomic implications. Biologia 67(5): 867–874. https://doi.org/10.2478/s11756-012-0076-z
- Khosroshahi EE, Salmaki Y (2019) Evolution of trichome types and its systematic significance in the genus Phlomoides (Lamioideae-Lamiaceae). Nordic Journal of Botany 37(5): 40–48. https://doi.org/10.1111/njb.02132
- Li HW (1985) Labiatae. In: Wu CY (Ed.) Flora Xizangica (Vol. 4). Science Press, Beijing, 98–299.
- Li HW, Hedge IC (1994) Lamiaceae. In: Wu CY, Raven PH (Eds) Flora of China (Vol. 17). Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, 50–299.
- Mathiesen C, Scheen A-C, Lindqvist C (2011) Phylogeny and biogeography of the lamioid genus Phlomis (Lamiaceae). Kew Bulletin 66(1): 83–99. https://doi.org/10.1007/s12225-011-9257-0
- Miller MA, Pfeiffer W, Schwartz T (2010) . Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, 8 pp. https://doi.org/10.1109/GCE.2010.5676129
- Rambaut A (2014) FigTree, v.1.4.2. http://tree.bio.ed.ac.uk/software/figtree/
- Ronquist F, Teslenko M, Van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Salmaki Y, Zarre S, Heubl G (2012a) The genus Phlomoides Moench (Lamiaceae; Lamioideae; Phlomideae) in Iran: An updated synopsis. Iranian Journal of Botany 18(2): 207–219.
- Salmaki Y, Zarre S, Ryding O, Lindqvist C, Scheunert A, Bräuchler C, Günther H (2012b) Phylogeny of the tribe Phlomideae (Lamioideae: Lamiaceae) with special focus on Eremostachys and Phlomoides: New insights from nuclear and chloroplast sequences. Taxon 61(1): 161–179. https://doi.org/10.1002/tax.611012
- Scheen A-C, Bendiksby M, Ryding O, Mathiesen C, Albert VA, Lindqvist C (2010) Molecular phylogenetics, character evolution and suprageneric classification of Lamioideae (Lamiaceae). Annals of the Missouri Botanical Garden 97(2): 191–219. https://doi.org/10.3417/2007174
- Seyedi Z, Salmaki Y (2015) Trichome morphology and its significance in the systematics of Phlomoides (Lamiaceae; Lamioideae; Phlomideae). Flora 213: 40–48. https://doi.org/10.1016/j.flora.2015.04.003
- Stamatakis A (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Thiers B (2022) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden's Virtual Herbarium.
- Wu CY, Li HW, Chen J, Huang SQ, Li H, Fang RZ, Huang SH, Zhuang X, Li YR, Bai PY (1977) Lamiaceae. In: Wu CY (Ed.) Flora Yunnanica (Vol. 1). Science Press, Beijing, 497–817.
- Xiang CL, Dong ZH, Peng H, Liu ZW (2010) Trichome micromorphology of the East Asiatic genus Chelonopsis (Lamiaceae) and its systematic implications. Flora 205(7): 434–441. https://doi.org/10.1016/j.flora.2009.12.007
- Xiang CL, Dong HJ, Hu GX, Zhou J, Peng H (2014) Taxonomic notes on the genus Phlomoides (Lamiaceae: Lamioideae) from China. Plant Diversity Resources 36: 551–560.
- Yao G, Deng YF, Ge XJ (2013) Validation of the name Pogostemon petelotii (Lamiaceae). Phytotaxa 81(1): 38–44. https://doi.org/10.11646/phytotaxa.81.1.11
- Zhao F, Chen YP, Salmaki Y, Drew BT, Wilson TC, Scheen A-C, Celep F, Bräuchler C, Bendiksby M, Wang Q, Min DZ, Peng H, Olmstead RG, Li B, Xiang CL (2021) An updated tribal classification of Lamiaceae based on plastome phylogenomics. BMC Biology 19(1): 1–2. https://doi.org/10.1186/s12915-020-00931-z
- Zhao Y, Chen YP, Liu DT, Turginov OT, Liang CZ, Xiang CL (2021a) The identity of Phlomoides pararotata (Lamiaceae, Lamioideae). Phytotaxa 524(2): 107–113. https://doi.org/10.11646/phytotaxa.524.2.5
- Zhao Y, Chen YP, Zhen HL, Peng H, Xiang CL (2021b) Phlomoides liangwangshanensis (Lamiaceae), a new species from Yunnan, China. Phytotaxa 491(1): 72–78. https://doi.org/10.11646/phytotaxa.491.1.8
- Zhao Y, Chen YP, Yuan JC, Paton AJ, Nuraliev MS, Zhao F, Drew BT, Salmaki Y, Turginov OT, Sun M, Sennikov AN, Yu XQ, Li B, Xiang CL (2023a) Museomics in Lamiaceae: Resolving the taxonomic mystery of Pseudomarrubium. Current Plant Biology 35(35–36): e100300. https://doi.org/10.1016/j.cpb.2023.100300
- Zhao Y, Zhao F, Salmaki Y, Paton A, Cai J, Drew BT, Chonour M, Efimov P, Turginov OT, Liang CZ, Chen YP, Xiang CL (2023b) Home at last: Molecular phylogenetic analyses support subsuming Metastachydium within Phlomoides (Lamiaceae). Taxon 72(3): 590–606. https://doi.org/10.1002/tax.12935
- Zhao Y, Chen YP, Drew BT, Zhao F, Almasi M, Turginov OT, Xiao JF, Salmaki Y, Yu XQ, Xiang CL (2024) (in press) Molecular phylogeny and taxonomy of Phlomoides (Lamiaceae subfamily Lamioideae) in China: Insights from molecular and morphological data. Plant Diversity.