Evaluating the Effects of Environmental Disturbances and Pesticide Mixtures on Soil Microbial Endpoints

These data and scripts are related to the article entitled "Evaluating the Effects of Environmental DIsturbances and Pesticide Mixtures on Soil Microbial Endpoints". The objectives of this study were to evaluate the effects of temperature and humidity disturbances and pesticide active ingredients exposure on soil microbial community structure and functions. To this end, 250 soil microcosms were exposed to either a heat disturbance, a high humidity to mimic heavy rain, or no environmental disturbance. After three days of recovery, soil microcosms were treated with different active ingredients: clopyralid (herbicide), cypermethrin (insecticide) and pyraclostrobin (fungicide). The treatments were applied alone or in combination at 1x or 10x of the agronomical dose. We then evaluated the effects of the disturbances and the active ingredients on various microbial endpoints related to the diversity and the structure of soil microbial communities, and with a specific focus on microbial guilds involved in nitrification. Data and codes needed to reproduce the statistical analyses presented in the manuscript are provided.