Single-nucleus RNA-sequencing processed data of normal adrenal cortex and benign adrenocortical tumors

Organism: Homo sapiens

Tissue: Adrenal cortex

Samples: 4 normal adrenal cortex (NAd) samples, 8 adrenocortical adenoma (ACA) samples, 6 primary bilateral macronodular adrenal hyperplasia (PBMAH) samples

Experimental design: After frozen tissue dissociation, nuclei were incubated with a Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody Mab414 (BioLegend), then sorted using a FACSAria III (BD Biosciences)  with the 85 μm nozzle and the BD FACSDIVA software. Sorted nuclei were immediately processed on a Chromium Controller (10x Genomics). Library preparation was performed using the Chromium Single-Cell v3 3′ Gel Bead, Chip and Library Kits (10x Genomics) according to the manufacturer’s protocol. Librairies were sequenced on a NextSeq 500 platform (Illumina) with paired-end sequencing.

Data processing: The demultiplexing, barcoded processing and gene counting were performed with the Cell Ranger software v3.1.0.

File format and content: The dataset includes the filtered expression matrices generated by Cell Ranger for individual samples. Each filtered expression matrix is saved as 3 files (***_barcodes.tsv.gz, ***_features.tsv.gz, and ***_matrix.mtx.gz) where *** is replaced by the sample name.