Dataset related to the article "N-acetylcysteine Amide AD4/NACA and Thioredoxin Mimetic Peptides Inhibit Platelet Aggregation and Protect against Oxidative Stress"

This record contains raw data related to the article "N-acetylcysteine Amide AD4/NACA and Thioredoxin Mimetic Peptides Inhibit Platelet Aggregation and Protect against Oxidative Stress"

Abstract: In the present study, we tested the effect of small-molecular-weight redox molecules on
collagen-induced platelet aggregation. We used N-acetylcysteine amide (AD4/NACA), the amide
form of N-acetylcysteine (NAC), a thiol antioxidant with improved lipophilicity and bioavailability
compared to NAC, and the thioredoxin-mimetic (TXM) peptides, TXM-CB3, TXM-CB13, and
TXM-CB30. All compounds significantly inhibited platelet aggregation induced by collagen, with
TXM-peptides and AD4 being more effective than NAC. The levels of TxB2 and 12-HETE, the main
metabolites derived from the cyclooxygenase and lipoxygenase pathways following platelet activation,
were significantly reduced in the presence of AD4, TXM peptides, or NAC, when tested at the
highest concentration (0.6 mM). The effects of AD4, TXM-peptides, and NAC were also tested on
the clotting time (CT) of whole blood. TXM-CB3 and TXM-CB30 showed the greatest increase in CT.
Furthermore, two representative compounds, TXM-CB3 and NAC, showed an increase in the antioxidant
free sulfhydryl groups of plasma detected via Ellman’s method, suggesting a contribution
of plasma factors to the antiaggregating effects. Our results suggest that these small-molecularweight
redox peptides might become useful for the prevention and/or treatment of oxidative stress
conditions associated with platelet activation.