RNA-Seq ACSS2 KD BA

Cellular RNA was isolated using the RNeasy® Mini Kit (Qiagen, 74104). RNA quality was923
analysed and quantified via the Bioanalyzer (Agilent). 200 ng of total RNA was used for library924
preparation. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New925
England Biolabs GmbH, Germany) according to the manufacturer’s protocol. The quantity and926
quality of the libraries were assessed by BioAnalyzer (Agilent). Generated libraries were927
sequenced on an Illumina NovaSeq instrument with around 35 million reads per library (100928
bp, paired-end).