Dataset Open Access

Polarization measurements of DOC-dependent IpaB-IpaD interactions

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB28-226 construct was measured using fluorescence polarization. Here, the DOC effect on binding affinity between IpaB and the engineered IpaD π-helix mutants was quantified by holding IpaB28-226-Alexa568 concentration constant  while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 μM with identical conditions then tested in the presence of 1 mM DOC.

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