Published December 20, 2023
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A new cryptic species of terrestrial breeding frog of the Pristimantis danae Group (Anura, Strabomantidae) from montane forests in Ayacucho, Peru
Authors/Creators
- 1. Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos, Lima, Peru|Universidad Nacional Mayor de San Marcos, Lima, Peru
- 2. Florida International University, Miami, United States of America
Description
Based on morphological and molecular characters, we describe a new species of terrestrial breeding frog of the Pristimantis danae Group from montane forests of La Mar Province, Ayacucho Department in southern Peru, at elevations from 1200 to 2000 m a.s.l. The phylogenetic analysis, based on concatenated sequences of gene fragments of 16S rRNA, RAG1, COI and TYR suggests that the new species is a sister taxon of a clade that includes one undescribed species of Pristimantis from Cusco, Pristimantis pharangobates and Pristimantis rhabdolaemus. The new species is most similar to P. rhabdolaemus, which differs by lacking scapular tubercules and by its smaller size (17.0–18.6 mm in males [n = 5], 20.8–25.2 mm in females [n = 5] in the new species vs. 22.8–26.3 mm in males [n = 19], 26.0–31.9 mm in females [n = 30] of P. rhabdolaemus). Additionally, we report the prevalence of Batrachochytrium dendrobatidis (Bd) in this species.
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References
- Acevedo AA, Armesto O, Palma E (2020) Two new species of Pristimantis (Anura: Craugastoridae) with notes on the distribution of the genus in northeastern Colombia. Zootaxa 4750: 499–523. https://doi.org/10.11646/zootaxa.4750.4.3
- Aguilar C, Wood Jr PL, Cusi JC, Guzman A, Huari F, Lundberg M, Mortensen E, Ramirez C, Robles D, Suarez J, Ticona A, Vargas VJ, Venegas PJ, Sites Jr JW (2013) Integrative taxonomy and preliminary assessment of species limits in the Liolaemus walkeri complex (Squamata, Liolaemidae) with descriptions of three new species from Peru. ZooKeys 47(364): 47–91. https://doi.org/10.3897/zookeys.364.6109
- Berger L, Speare R, Daszak P, Green DE, Cunningham AA, Goggin CL, Scolombe R, Ragan MA, Hyatt AD, McDonald K, Hines HB, Lips KR, Maranteli G, Parkes H (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Sciences of the United States of America 95(15): 9031–9036. https://doi.org/10.1073/pnas.95.15.9031
- Bossuyt F, Milinkovitch MC (2000) Convergent adaptive radiations in Madagascan and Asian ranid frogs reveal covariation between larval and adult traits. Proceedings of the National Academy of Sciences of the United States of America 97: 6585–6590. https://doi.org/10.1073/pnas.97.12.6585
- Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms 60(2): 141–148. https://doi.org/10.3354/dao060141
- Briggs JM, Knapp AK, Blair JM, Heisler JL, Hoch GA, Lett MS, McCarron JK (2005) An ecosystem in transition: Causes and consequences of the conversion of mesic grassland to shrubland. Bioscience 55(3): 243–254. https://doi.org/10.1641/0006-3568(2005)055[0243:AEITCA]2.0.CO;2
- Carrión-Olmedo JC, Ron SR (2021) A new cryptic species of the Pristimantis lacrimosus group (Anura, Strabomantidae) from the eastern slopes of the Ecuadorian Andes. Evolutionary Systematics 5: 151–175. https://doi.org/10.3897/evolsyst.5.62661
- Catenazzi A (2015) State of the world's amphibians. Annual Review of Environment and Resources 40(1): 91–119. https://doi.org/10.1146/annurev-environ-102014-021358
- Catenazzi A, Vredenburg VT, Lehr E (2010) Batrachochytrium dendrobatidis in the live frog trade of Telmatobius (Anura: Ceratophryidae) in the tropical Andes. Diseases of Aquatic Organisms 92(2–3): 187–191. https://doi.org/10.3354/dao02250
- Catenazzi A, Lehr E, Rodriguez LO, Vredenburg VT (2011) Batrachochytrium dendrobatidis and the collapse of anuran species richness and abundance in the upper Manu National Park, southeastern Peru. Conservation Biology 25(2): 382–391. https://doi.org/10.1111/j.1523-1739.2010.01604.x
- Dayrat B (2005) Towards integrative taxonomy. Biological Journal of the Linnean Society, Linnean Society of London 85(3): 407–417. https://doi.org/10.1111/j.1095-8312.2005.00503.x
- De Queiroz K (2005) Ernst Mayr and the modern concept of species. Proceedings of the National Academy of Sciences 102(suppl_1): 6600–6607. https://doi.org/10.1073/pnas.0502030102
- Duellman WE (1978) Two New Species of Eleutherodactylus (Anura: Leptodactylidae) from the Peruvian Andes. Transactions of the Kansas Academy of Science (1903) 81(1): 65–71. https://doi.org/10.2307/3627358
- Duellman WE, Lehr E (2009) Terrestrial-breeding frogs (Strabomantidae) in Peru. Natur und Tier Verlag.
- Duellman WE, Lehr E, Venegas PJ (2006) Two new species of Eleutherodactylus (Anura: Leptodactylidae) from the Andes of northern Peru. Zootaxa 1285(1): 51–64. https://doi.org/10.11646/zootaxa.1512.1.3
- Elmer KR, Cannatella DC (2008) Three new species of leaflitter frogs from the upper Amazon forests: cryptic diversity within Pristimantis ockendeni (Anura: Strabomantidae) in Ecuador. Zootaxa 1784(1): 11–38. https://doi.org/10.11646/zootaxa.1784.1.2
- Faivovich J, Haddad CF, Garcia PC, Frost DR, Campbell JA, Wheeler WC (2005) Systematic review of the frog family Hylidae, with special reference to Hylinae: Phylogenetic analysis and taxonomic revision. Bulletin of the American Museum of Natural History 2005(294): 1–240. https://doi.org/10.1206/0003-0090(2005)294[0001:SROTFF]2.0.CO;2
- Frost DR (2023) Amphibian Species of the World: an Online Reference. Version 6.2 (30 March 2023). Electronic Database accessible at https://amphibiansoftheworld.amnh.org/index.php. American Museum of Natural History, New York, USA. https://doi.org/10.5531/db.vz.0001
- Guayasamin JM, Hutter CR, Tapia EE, Culebras J, Peñafiel N, Pyron RA, Morochz C, Funk WC, Arteaga A (2017) Diversification of the rainfrog Pristimantis ornatissimus in the lowlands and Andean foothills of Ecuador. PLoS ONE 12(3): e0172615. https://doi.org/10.1371/journal.pone.0172615
- Hazzi NA, Moreno JS, Ortiz-Movliav C, Palacio RD (2018) Biogeographic regions and events of isolation and diversification of the endemic biota of the tropical Andes. Proceedings of the National Academy of Sciences of the United States of America 115(31): 7985–7990. https://doi.org/10.1073/pnas.1803908115
- Hedges SB, Duellman WE, Heinicke MP (2008) New World direct-developing frogs (Anura: Terrarana): molecular phylogeny, classification, biogeography, and conservation. Zootaxa 1737(1): 1–182. https://doi.org/10.11646/zootaxa.1737.1.1
- Heinicke MP, Duellman WE, Hedges SB (2007) Major Caribbean and Central American frog faunas originated by ancient oceanic dispersal. Proceedings of the National Academy of Sciences of the United States of America 104(24): 10092–10097. https://doi.org/10.1073/pnas.0611051104
- Herrera-Alva V, Díaz V, Castillo-Urbina E, Rodolfo C, Catenazzi A (2020) A new species of Atelopus (Anura: Bufonidae) from southern Peru. Zootaxa 4853(3): 404–420. https://doi.org/10.11646/zootaxa.4853.3.4
- Hillis DM (2019) Species delimitation in herpetology. Journal of Herpetology 53(1): 3–12. https://doi.org/10.1670/18-123
- Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17(8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
- Hutter CR, Guayasamin JM (2015) Cryptic diversity concealed in the Andean cloud forests: Two new species of rainfrogs (Pristimantis) uncovered by molecular and bioacoustic data. Neotropical Biodiversity 1(1): 36–59. https://doi.org/10.1080/23766808.2015.1100376
- Hyatt AD, Boyle DG, Olsen V, Boyle DB, Berger L, Obendorf D, Dalton A, Kriger K, Hero M, Hines H, Phillott R, Campbell R, Marantelli G, Gleason F, Colling A (2007) Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms 73: 175–192. https://doi.org/10.3354/dao073175
- Kieswetter CM, Schneider CJ (2013) Phylogeography in the northern Andes: Complex history and cryptic diversity in a cloud forest frog, Pristimantis w-nigrum (Craugastoridae). Molecular Phylogenetics and Evolution 69(3): 417–429. https://doi.org/10.1016/j.ympev.2013.08.007
- Köhler J, Castillo-Urbina E, Aguilar-Puntriano C, Vences M, Glaw F (2022) Rediscovery, redescription and identity of Pristimantis nebulosus (Henle, 1992), and description of a new terrestrial-breeding frog from montane rainforests of central Peru (Anura, Strabomantidae). Zoosystematics and Evolution 98(2): 213–232. https://doi.org/10.3897/zse.98.84963
- Lanfear R, Calcott B, Ho SYW, Guindon S (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29: 1695–1701. https://doi.org/10.1093/molbev/mss020
- Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B (2017) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34(3): 772–773. https://doi.org/10.1093/molbev/msw260
- Lehr E (2007) New eleutherodactyline frogs (Leptodactylidae: Pristimantis, Phrynopus) from Peru. Bulletin of the Museum of Comparative Zoology 159(2): 145–178. https://doi.org/10.3099/0027-4100(2007)159[145:NEFLPP]2.0.CO;2
- Lehr E, Catenazzi A (2008) A new species of Bryophryne (Anura: Strabomantidae) from southern Peru. Zootaxa 1784(1): 1–10. https://doi.org/10.11646/zootaxa.1784.1.1
- Lehr E, Catenazzi A (2009) Three new species of Bryophryne (Anura: Strabomantidae) from the region of Cusco, Peru. South American Journal of Herpetology 4(2): 125–138. https://doi.org/10.2994/057.004.0204
- Lehr E, Catenazzi A (2010) Two new species of Bryophryne (Anura: Strabomantidae) from high elevations in southern Peru (Region of Cusco). Herpetologica 66(3): 308–319. https://doi.org/10.1655/09-038.1
- Lehr E, von May R (2017) A new species of terrestrial-breeding frog (Amphibia, Craugastoridae, Pristimantis) from high elevations of the Pui Pui Protected Forest in central Peru. ZooKeys 17(660): 17–42. https://doi.org/10.3897/zookeys.660.11394
- Lehr E, Moravec J, Cusi JC, Gvoždík V (2017a) A new minute species of Pristimantis (Amphibia: Anura: Craugastoridae) with a large head from the Yanachaga-Chemillén National Park in central Peru, with comments on the phylogenetic diversity of Pristimantis occurring in the Cordillera Yanachaga. European Journal of Taxonomy 325: 1–22. https://doi.org/10.5852/ejt.2017.325
- Lehr E, Von May R, Moravec J, Cusi JC (2017b) Three new species of Pristimantis (Amphibia, Anura, Craugastoridae) from upper montane forests and high Andean grasslands of the Pui Pui Protected Forest in central Peru. Zootaxa 4299(3): 301–336. https://doi.org/10.11646/zootaxa.4299.3.1
- Lips KR, Brem F, Brenes R, Reeve JD, Alford RA, Voyles J, Carey C, Livo L, Pessier A, Collins JP (2006) Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community. Proceedings of the National Academy of Sciences of the United States of America 103(9): 3165–3170. https://doi.org/10.1073/pnas.0506889103
- Lips KR, Diffendorfer J, Mendelson JR III, Sears MW (2008) Riding the wave: Reconciling the roles of disease and climate change in amphibian declines. PLoS Biology 6(3): e72. https://doi.org/10.1371/journal.pbio.0060072
- Lynch JD, Duellman WE (1997) Frogs of the genus Eleutherodactylus (Leptodactylidae) in western Ecuador: systematic, ecology, and biogeography. Natural History Museum, University of Kansas, 252 pp. https://doi.org/10.5962/bhl.title.7951
- Lynch JD, McDiarmid R (1987) Two new species of Eleutherodactylus (Amphibia: Anura: Leptodactylidae) for Bolivia. Proceedings of the Biological Society of Washington 100: 337–346. https://doi.org/10.2307/1447850
- Meyer CP (2003) Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics. Biological Journal Linnean Society 79: 401–459. https://doi.org/10.1046/j.1095-8312.2003.00197.x
- Minoli I, Morando M, Avila LJ (2014) Integrative taxonomy in the Liolaemus fitzingerii complex (Squamata: Liolaemini) based on morphological analyses and niche modeling. Zootaxa 3856(4): 501–528. https://doi.org/10.11646/zootaxa.3856.4.3
- Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268–274. https://doi.org/10.1093/molbev/msu300
- Ortega-Andrade HM, Rojas-Soto OR, Valencia JH, Espinosa de los Monteros A, Morrone JJ, Ron SR, Cannatella DC (2015) Insights from integrative systematics reveal cryptic diversity in Pristimantis frogs (Anura: Craugastoridae) from the Upper Amazon Basin. PLoS ONE 10(11): e0143392. https://doi.org/10.1371/journal.pone.0143392
- Padial JM, De la Riva I (2009) Integrative taxonomy reveals cryptic Amazonian species of Pristimantis (Anura: Strabomantidae). Zoological Journal of the Linnean Society 155(1): 97–122. https://doi.org/10.1111/j.1096-3642.2008.00424.x
- Padial JM, Castroviejo-Fisher S, Köhler J, Domic E, De la Riva I (2007) Systematics of the Eleutherodactylus fraudator species group (Anura: Brachycephalidae). Herpetological Monograph 21(1): 213–240. https://doi.org/10.1655/06-007.1
- Padial JM, Taran G, Frost DR (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa 3825(1): 1–132. https://doi.org/10.11646/zootaxa.3825.1.1
- Páez NB, Ron SR (2019) Systematics of Huicundomantis, a new subgenus of Pristimantis (Anura, Strabomantidae) with extraordinary cryptic diversity and eleven new species. ZooKeys 868: 1–112. https://doi.org/10.3897/zookeys.868.26766
- Pinto-Sánchez NR, Ibáñez R, Madriñán S, Sanjur OI, Bermingham E, Crawford AJ (2012) The great American biotic interchange in frogs: multiple and early colonization of Central America by the South American genus Pristimantis (Anura: Craugastoridae). Molecular Phylogenetics and Evolution 62(3): 954–972. https://doi.org/10.1016/j.ympev.2011.11.022
- Pyron RA, Wiens JJ (2011) A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution 61(2): 543–583. https://doi.org/10.1016/j.ympev.2011.06.012
- Reyes-Puig C, Mancero E (2022) Beyond the species name: An analysis of publication trends and biases in taxonomic descriptions of rainfrogs (Amphibia, Strabomantidae, Pristimantis). ZooKeys 1134: 73–100. https://doi.org/10.3897/zookeys.1134.91348
- Rojas RR, Fouquet A, Ron SR, Hernández-Ruz EJ, Melo-Sampaio PR, Chaparro JC, Vogt RC, Tadeus De Carvalho V, Cardoso Pinheiro L, Avila R, Pires A, Gordo M, Hrbek T (2018) A Pan-Amazonian species delimitation: high species diversity within the genus Amazophrynella (Anura: Bufonidae). PeerJ 6: e4941. https://doi.org/10.7717/peerj.4941
- Scheele BC, Pasmans F, Berger L, Skerratt LF, Martel A, Beukema W, Acevedo AA, Burrowes PA, Carvhalo T, Catenazzi A, De la Riva I, Fisher MC, Flechas S, Foster C, Frías-Alvarez P, Garner TWJ, Gratwicke B, Guayasamin JM, Hirschfeld M, Kolby JE, Kosch TA, La Marca E, Lindenmayer DB, Lips KR, Longo AV, Maneyro R, McDonald CA, Mendelson J, Palacios-Rodriguez P, Parra-Olea G, Richards-Zawacki CL, Rödel M, Rovito SM, Soto-Azat C, Toledo LF, Voyles J, Weldon C, Whitfield SM, Wilkinson M, Zamudio KR, Canessa S (2019) The aftermath of an amphibian fungal panzootic reveals unprecedented loss of biodiversity. Science 636: 1459–1463. https://doi.org/10.1126/science.aav0379
- Schlick-Steiner BC, Steiner FM, Seifert B, Stauffer C, Christian E, Crozier RH (2010) Integrative taxonomy: A multisource approach to exploring biodiversity. Annual Review of Entomology 55(1): 421–438. https://doi.org/10.1146/annurev-ento-112408-085432
- Simpson GG (1961) Principles of animal taxonomy. In Principles of Animal Taxonomy. Columbia University Press, 250 pp. https://doi.org/10.7312/simp92414
- Siqueira S, Aguiar Jr O, Souza MB, Lima AP, Recco‐Pimentel SM (2009) Unusual intra‐individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana). Hereditas 146(4): 141–151. https://doi.org/10.1111/j.1601-5223.2009.02104.x
- Swenson JJ, Young BE, Beck S, Comer P, Córdova JH, Dyson J, Embert D, Encarnación F, Ferreira W, Franke I, Grossman D, Hernadez P, Herzog SK, Josse C, Navarro G, Pacheco V, Stein BA, Timaná M, Tovar A, Tovar C, Vargas J, Zambrana-Torrelio CM (2012) Plant and animal endemism in the eastern Andean slope: Challenges to conservation. BMC Ecology 12(1): 1–19. https://doi.org/10.1186/1472-6785-12-1
- Tamura K, Glen S, Sudhir K (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38(7): 3022–3027. https://doi.org/10.1093/molbev/msab120
- von May R, Catenazzi A, Corl A, Santa-Cruz R, Carnaval AC, Moritz C (2017) Divergence of thermal physiological traits in terrestrial breeding frogs along a tropical elevational gradient. Ecology and Evolution 7(9): 3257–3267. https://doi.org/10.1002/ece3.2929
- Waddell EH, Crotti M, Lougheed SC, Cannatella DC, Elmer KR (2018) Hierarchies of evolutionary radiation in the world's most species rich vertebrate group, the Neotropical Pristimantis leaf litter frogs. Systematics and Biodiversity 16(8): 807–819. https://doi.org/10.1080/14772000.2018.1503202
- Warne RW, LaBumbard B, LaGrange S, Vredenburg VT, Catenazzi A (2016) Co-Infection by Chytrid Fungus and Ranaviruses in Wild and Harvested Frogs in the Tropical Andes. PLoS ONE 11(1): e0145864. https://doi.org/10.1371/journal.pone.0145864
- Wilcox TP, Zwickl DJ, Heath TA, Hillis DM (2002) Phylogenetic relationships of the dwarf boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution 25: 361–371. https://doi.org/10.1016/S1055-7903(02)00244-0
- Wiley EO (1978) The evolutionary species concept reconsidered. Systematic Zoology 27(1): 17–26. https://doi.org/10.2307/2412809
- Zozaya SM, Higgie M, Moritz C, Hoskin CJ (2019) Are pheromones key to unlocking cryptic lizard diversity? American Naturalist 194(2): 168–182. https://doi.org/10.1086/704059