Planktonic Food Web Structure at a Coastal Site

Fluorescent Labeled Bacteria (FLB) Uptake Experiment- whole (unfiltered) seawater (WSW) was collected on the HOE Legacy 2A cruise near station ALOHA, Hawaii. Water was collected from the Niskin into 3, 2.3L bottles. FLBs were added to the bottles, and 100ml was removed and preserved from each bottle at T0. Bottles were incubated on-deck for 1h, at which point another 100ml was preserved from each bottle. Preserved samples were filtered onto 2.0um blackened polycarbonate filters and stained with DAPI. Slides were stored at -20C.