Published March 6, 2024 | Version v1
Dataset Open

Light beads microscopy recordings of up to one million neurons across mouse dorsal cortex during spontaneous behavior

Authors/Creators

  • 1. Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA
  • 2. The Kavli Neural Systems Institute, The Rockefeller University, New York, NY, USA

Description

Example datasets and source code accompanying the article:

Manley, J., Lu, S., Barber, K., Demas, J., Kim, H., Meyer, D., Martínez Traub, F., & Vaziri, A. (2024). Simultaneous, cortex-wide dynamics of up to 1 million nuerons reveal unbounded scaling of dimensionality with neuron number. Neuron. https://doi.org/10.1016/j.neuron.2024.02.011.

Provided are example recordings of neuronal calcium dynamics across mouse cortex, captured utilizing Light Beads Microscopy (as described in Demas et al. 2021). All behavior and neural activity are spontaneous, i.e. the mouse was kept in the dark and not presented with any experimental stimuli. Neural activity was extracted using the CaImAn-based pipeline provided at https://github.com/vazirilab/MAxiMuM_processing_tools.

The following software packages are provided in .zip files in their state at the time of publication. However, it is highly recommended to utilize the repositories at https://github.com/vazirilab in case of future updates:

 

See README.md for additional details.

Notes

This research was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award numbers 1U01NS115530, 5U01NS103488 and 1U01NS126057, and the Kavli Foundation through the Kavli Neural Systems Institute.

Files

README.md

Files (79.8 GB)

Name Size
md5:7247f0c31b32da45a2971e4919a49e26
30.7 GB Download
md5:5fd768446d564984702c870235e0f557
22.3 GB Download
md5:65fda7e26be520874de3227914530b89
11.1 GB Download
md5:af6e1358ee7a5d86678d06532ec72b9b
14.4 GB Download
md5:1473aa30f734ae92f48ece41592185b1
1.2 GB Download
md5:5271e929f70bb36929c8024c76cab96a
15.8 kB Preview Download
md5:cfda0b6a10362fe9b5f84281e2416d15
4.6 kB Preview Download
md5:941434380c4f6c9359167ef72d0f5dc8
14.9 MB Preview Download
md5:c37b90d258f1bb5cbae4cbffcf6794ba
3.8 kB Preview Download

Additional details

Related works

Is supplement to
Journal article: 10.1016/j.neuron.2024.02.011 (DOI)
Preprint: 10.1101/2024.01.15.575721 (DOI)

Funding

National Institute of Neurological Disorders and Stroke
Optimization, application and dissemination of high-speed hybrid multiphoton volumetric imaging technologies 1U01NS115530
National Institute of Neurological Disorders and Stroke
Cortex-wide volumetric imaging of neuronal activity 5U01NS103488
National Institute of Neurological Disorders and Stroke
Optimization, application, and dissemination of imaging modules for high-speed mesoscopic volumetric recording of neuroactivity in scattering brains 1U01NS126057
The Kavli Foundation

Software

Repository URL
https://github.com/vazirilab/scaling_analysis
Programming language
Python