Isolation and Characterization of Pathogenic Bacteria in Some Samples in Governorate Homs

In the framework of this work, 13 samples of well water were collected from the city of Homs (Al-Arman Al-Janoubi, two samples from Wadi Al-Dahab, Al-Zahra), and from the countryside (Al-Jabriya, Al-Awar, two samples from Zaidal, Qatina, Al-Dar Al-Kabira, Jandar, Al-Mushrifa, Al-Madabae) with the aim of detecting the presence of  Salmonella, Shigella, E.coli, Klebsiella,  Pseudomonas, and Listeria bacteria in well water in Homs Governorate and its countryside, and diagnosing them based on a set of cultural and microscopic characteristics and some biochemical tests through the use of the three-test method (probabilistic, confirmatory and complementary test ) and the membrane filter method. The results of the  probabilistic test for well water samples taken from (Al-Jabriya, Al-Awar, two samples from Zaidal, Qatina, Al-Dar Al-Kabira, two samples from Wadi Al-Dahab, Al-Mushrifa, Al-Madabae, and Al-Zahra) showed the appearance of gas in the Durham tubes after 48 hours of incubation, which indicates the possibility of contamination bacteria belonging to the coliform group, and the result of the confirmatory and complementary test was the appearance of smooth, metallic-glossy colonies on eosin medium, methylene blue short, gram-negative bacilli belonging to E.coli, which indicates that it is not suitable for drinking and irrigating crops. In the samples of Jandar and Al-Arman Al-Janoubi, no gas appeared after 48 hours of incubation, which indicates that they are suitable for drinking and irrigating crops. When using the membrane filtration method, it was found that all studied samples were contaminated with the genus Klebsiella, except for the samples of Jandar and Al-Arman Al-Janoubi, which were also found to be free of pathogenic bacteria Salmonella, Shigella, Pseudomonas, Listeria, unlike the rest of the studied samples.

Key words:

 well water, pathogenic bacteria, membrane filters, three tests method, Homs.