SYSTEMATIC STANDARDIZATION AND PHYSIOCHEMICAL EVALUATION OF NOVEL SIDDHA FORMULATION PUNGAMPOO CHOORANAM AS PER AYUSH GUIDELINES BY MODERN ANALYTICAL TECHNIQUES

Siddha system of medicine is one of the oldest Indian system of medicine known to the mankind. Several indigenous siddha formulations formulated and hypothesized by ancient siddha practitioners are still used for clinical management of several dreadful metabolic disorders in humans like diabetes mellitus. Global market for siddha formulations seems remarkable but the need of the hour is to ensure the standard of the preparation. Starting from raw material to finished product the quality and genuinity of the product as to be ascertained. Considering the global need the modern standardization method adopted for identity, purity and shelf life of the preparation. Different techniques has been followed to analyze the purity of the raw materials it includes microscopic, macroscopic, Physical, chemical and biological method of analysis. Hence the main aim of the present investigation is to standardize the novel formulation Pungampoo Chooranam (PPC) as per AYUSH guideline and to reveal the property of the formulation to the scientific community for better understanding about the standards of the formulation. The results obtained from the physicochemical evaluation revels that the total ash value of PPCwas found to 8.01 %. In which the water soluble ash was 4.75% and acid insoluble ash was 0.69%. Similarly loss on drying value at 105^oC was fond to be 9.24% respectively. The water soluble extractive value of PPC was found to be 26.46 % whereas alcohol soluble extractive value is 29.35 %. n-hexane soluble extractive value of the test drug PPC was found to be 19.26%. Preliminary TLC analysis of the sample PPC emits fluorescence indicates the presence of fluorescent emitting compound. The results of HPTLC analysis of the sample PPC reveals the presence of 14 prominent peaks corresponds to 14 different compound’s with Rf value ranging from 0.01 to 0.93 with percentage area of 0.82 to 18.52%. From the results of the present investigation it was concluded that the formulation PPC is highly stable and also possess biologically significant phytocomponents which is may be used as an ailment for treating various disease