Phasor Dataset generated at NEST (Scuola Normale Superiore)

The folder contains sub-folders used in the work "Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks" by M.Bernardi and F.Cardarelli. The data served for specific examples on Phasor-FLIM customary analysis within the framework of the Python Notebook ran in Google Colab, extensively documented and available at: https://github.com/Mariochem92/PhasorIdentifier

Irinotecan and its metabolite SN-38 in solution at different pH

File format: .R64

FLIM Acquisition: DFD-FLIM 

Laser repetition frequency: 80 MHz

Instrumentation: Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)

Can be used to test any functionality in the analysis of evolutions within the phasor plot. Specially designed for pH analysis within the pH range of 2 to 12.
Reference: Bernardi et al., A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks, 2023

Encapsulated irinotecan in commercial liposomal nanoformulation Onivyde 

File format: .R64

FLIM Acquisition: DFD-FLIM 

Laser repetition frequency: 80 MHz

Instrumentation: Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)

Can be used to test any functionality in the analysis of the supramolecular organization. 

Reference: Bernardi et al., Fluorescence Lifetime Nanoscopy of Liposomal Irinotecan Onivyde: From Manufacturing to Intracellular Processing, ACS Appl. Bio Mater., 2023

INS1-E cells upon cytokine treatment

File format: .R64

FLIM Acquisition: DFD-FLIM 

Laser repetition frequency: 80 MHz

Instrumentation: Olympus FVMPE-RS with FLIM box system (ISS, Urbana Champaign)

INS1E control and INS1E cytokines, can be used to test any functionality in the analysis of the supramolecular organization. 
Reference: Pugliese et al, Unveiling nanoscale optical signatures of cytokine-induced β-cell dysfunction, Sci Rep, 2023

Encapsulated doxorubicin 

File format: .ref

FLIM Acquisition: TD-FLIM 

Laser repetition frequency: 40 MHz

Instrumentation: Leica TCS SP5 confocal microscope

Can be used to test any functionality in the analysis of the supramolecular organization and signal evolution. Specially designed for storage analysis at temperature 4°C, 25°C and 37°C.
Reference: Carretta et al, Monitoring drug stability by label-free fluorescence lifetime imaging: a case study on liposomal doxorubicin. ,J. Phys. : Conf Ser, 2023