KSR inhibitor APS-2-79 sensitivity test in JURKAT and ALL-SIL T-cell acute lymphoblastic leukemia cell lines measured by Cell Counting Kit 8

APS-2-79 compound was purchased from MedChemExpress (Monmoutyh Junction, NJ, USA). The 20 mg/ml stock solution was prepared in DMSO. To calculate the IC50, JURKAT and ALL-SIL cells were cultured for 72h with a range of APS-2-79 concentrations (5-15 µM) added as equal volumes. Cells treated with 0.5% DMSO (vehicle) were used as negative control. Cells treated with 10% DMSO were used as positive control. The viability of cells was measured using Cell Counting Kit 8 (Sigma Aldrich) and GloMax Microplate Reader system (Promega) with 450 nm wavelength and 600 nm as reference wavelength. The relevant reads are made from following wells: 2A-2D (15 µM APS-2-79), 3A-3D (12.5 µM APS-2-79), 4A-4D (10 µM APS-2-79), 5A-5D (7.5 µM APS-2-79), 6A-6D (5 µM APS-2-79), 7A-7D (vehicle), 8A-8D (positive control).