Standard Experimental Protocols for the Culture and Evaluation of Immortalized Cell Lines Derived from Acute Leukemia Patients

This document presents a collection of standardized experimental protocols applied in the culture and evaluation of immortalized cell lines derived from acute leukemia patient samples, encompassing mononuclear cell isolation, Giemsa staining, and cell proliferation monitoring. The protocols are designed to ensure reproducibility and methodological consistency under Biosafety Level II laboratory conditions, in support of research.