IN VITRO ANTIOXIDANT EVALUATION OF FLOWRES OF EHRETIA LAEVIS ROXB BY DPPH RADICAL SCAVENGING ASSAY AND NIRIC OXIDE RADICAL SCAVENGING ASSAY

Ehretia laevis, a medicinal plant belonging to the boraginaceae family, is widely recognized for its therapeutic properties in traditional medicine. the present study aimed to explore the phytochemical profile and antioxidant potential of Ehretia laevis through hydro alcoholic extraction and solvent fractionation. The plant material was subjected to hydroalcoholic extraction (70% ethanol), followed by successive fraction into n-hexane, ethyl acetate, and tannins. Anti-oxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) and nitic oxide scavenging assays. The hydro alcoholic extract and ethyl acetate fraction demonstated significant free radical scavenging activity, indicating a high antioxidant potential, while the n-hexane and aqueous fraction is particularly enriched with potent antioxidant compounds. The results collectively validate the ethnomedicial relevance of ehretia laevis and provide a basis for its further pharmacological exploration.

Keywords: EHRETIA LAEVIS, Antioxidant Potential, DPPH (2,2-Diphenyl-1-Picrylhydrazyl) and Nitic oxide Scavenging assays.