Global Animal Science Journal
Published January 1, 2015 | Version v1
Journal article Open

Prevalence and characterization of Esherichia coli isolated from apparently healthy and diseased Cockatiles and budgerigars

Creators

  • 1. Bacteriology, Mycology and Immunology Dept. Faculty of Vet. Medicine, Suez Canal University, Egypt
  • 2. Wildlife Management and Zoo Medicine Faculty of Vet. Medicine, Suez Canal University, Egypt
  • 3. Bacteriology Veterinary serum and Vaccine Research institute Abbassia, Cairo, Egypt

Description

A total of 258 fecal samples from Cockatiles and budgerigars (230 from apparently healthy birds and 28 from clinically diseased) were collected. The bacteriological examination revealed the isolation of E.coli (51.28%), Salmonella (10.26%), Klebsiella (17.95%), Proteus (17.95%) and Enterobacter (2.56%) respectively in relation to total number of isolates. They were infected with percentage of E.coli (7.75%), Salmonella (1.55%), Klebsiella (2.71%), proteus (2.71%) and Enterobacter (0.39%) respectively in relation to total number of collected samples. The isolated E.coli serovars were belonging to O1 (13.3%), O2 (20%), O26 (6.7%) and O untypable (60%). E.coli strains were then examined for enterotoxin production, E.coli O26 and Ountypable was heat labile toxin producer [LT]. While O1, O2, Ountypable were verotoxin producer. On the other hand, no heat stable toxin producer strains [ST] were detected. RAPD PCR profile was used for differentiation between E.coli different serotypes and revealed a significant difference among the revealed serotypes. The antibiotic sensitivity tests revealed that, ciprofloxacin and gentamycin were the most effective drugs against the isolated E.coli.

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References

  • Baker, J.R. (1996): Causes of mortality and morbidity in exhibition budgerigars in the United Kingdom. Vet. Rec. Aug. 17,139: 156-162.
  • Char, N.L. and Rao, M. R. (1991): Zoonotically significant serotypes of E.coli isolated from pathologically condition of animal and poultry. Indian Vet. J. 68(11): 1003-1006.
  • Darrel, K.; Styles and Flammer K. (1991): Congo red binding of Escherichia coli isolated from the cloaca of psittacine birds. Avian diseases 35:46-48.
  • De Rycke, J.; Guillot, J.F. and Bolvin, R. (1987): Cytotoxins in non-enterotoxogenic strains of Escherichia coli isolated from feces of diarrheic calves. Vet. Microbiol., 15:137-150.
  • Dean, A.G.; Ching, Y.p; Willams, R.G.; and Harden, L.B. (1972): Test for Escherichia coli enterotoxin using infant mice application in a study of diarrhea children in Honolulu. J. Infec. Dis., 125:407-411.
  • Donta, S. T.; Moon, H.W. and Whipp, S.C. (1974): Detection of heat-labile Escherichia coli entero-toxins with the use of adrenal cells in tissue cultures. Science. 183:334-336.
  • Emery, D.A.; Nagaraja, K.V.; Shaw, D.P.; Newman, J.A. and White, D.G. (1992): Virulence factors of Escherichia coli associated with colisepticemia in chickens and turkeys. Avian, Dis., 36, 504-511.
  • Enas, M.S.A. (2008): Studies upon some bacterial isolates affecting budgerijars M.V.Sc., (Wild life and Zoo Medicine). Fac. Vet. Med., Suez Canal University.
  • Finegold, S.M. and Martin, W.J. (1982): Diagnostic Microbiology, 6
  • Th Ed., C.V. Mosby Co. St. Louis, Toronto, London.
  • Flammer, K. and Drewes, L. A. (1988): Species related differences in the incidence of gram-negative bacteria isolated from the cloaca of clinically normal psittacine birds. Avian Dis., 32: 79-83.
  • Forshaw, J. (1973) .Landsdowne Editions, Australia, 1973. William T Cooper (illustrator). 1st Edition.
  • Giannella, R. A. (1976): Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: Characteristics of the model. Infect. Immune. 14(1):95-99.
  • Graham, C.L. and Graham, D.L. (1978): Occurrence of E.coli in feces of psittacine birds. Avian Dis. 22: 717-720.
  • Krieg, N.R. and Holt, J.G. (1984):
  • Bergey’s Mannal of systematicology.
  • Vol. 1, William and Baltmore,
  • London.
  • Minsky L, Petrak ML, 1982. Diseases of the digestive system. In: Diseases of cage and aviary birds [ed. by Petrak, M. L.]. Philadelphia, Pennsylvania, USA: Lea & Febiger, 432-448.
  • Roy, P., Purushothaman, V.; Kotees-waran, A. and Dhillon, A.S. (2006): isolation Characterization and Antimicrobial Drug Resistance Pattern of Escherichia coli isolated from Japanese quail and their Environment. J. Appl. Poultres., 15:442-446.
  • Salehi, M. and Ghanbarpour, R. (2010): Phenotypic and genotypic properties of Escherichia coli isolated from colisepticemic cases of Japanese quail. Tropical Animal Health and Production J. Vol. 42, No. 7:1497-1504.
  • Sojka, W. J. 1965. Escherichia coli
  • in domestic animals and poultry.
  • Commonwealth Agricultural Bureaux,
  • Farnham Royal, UK.
  • Williams, J.G.K.; Kubelik, A.R.; Livak, K.J.; Rafalaski, J.A. and Tingey, S.V.(1990): “DNA polymor-phism amiplified by arbitrary primers are useful as useful as genetic markers”. Nucleic Acid Res., 18:6531-6535.