Global Animal Science Journal
Published January 1, 2013 | Version v1
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Molecular typing of the enteroaggregative E.coli heat-stable enterotoxin 1 gene (EAST1) in E. coli strains isolated from human and calves with diarrhea

Creators

  • 1. Department of Bacteriology, Mycology and Immunology .Faculty of veterinary medicine, Suez Canal University, Egyp
  • 2. Department of infectious disease control and international medicine, graduate school of medical and dental sciences, Niigata University Niigata, Japan

Description

A total of 76 E.coli strains isolated from human and calves with diarrhea were tested for the presence of the Enteroaggregative E.coli heat-stable enterotoxin 1 gene (EAST1) by using PCR then gene has been sequenced, followed by PCR detection of different virulence factors genes including shiga toxins 1 and 2, intimin, aggR, hemolysin, heat labile toxin, heat stable toxin, F5 and F5 genes. The EAST1 gene was found in most human strains accompanied with LT and ST gene in Enterotoxigenic strains (O6:H16), aggR (Fimbrial antigen-specific gene) in Enteroaggregative strains (O126:H27) and with shiga toxins and intimin genes in Enterohemorraghic strains (O157:H7 and O26:H11). Only 4 bovine strains (3 Enterotoxigenic strains and 1 enteropathogenic strain) with adherence factor F5 (K99) were also positive for the EAST1 gene. We conclouded that the presence of EAST1 gene not restricted to Enteroaggregative E.coli but also present in other pathotypes in combination with certain virulence marker.

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