Published September 29, 2023
| Version 1.0.0
Dataset
Open
16S V4-V4 taxonomy classifier
- 1. University of New Hampshire
- 2. Northern Gulf Institute and NOAA AOML
Description
16S metabarcoding databases and naive-bayes classifier specific to the V4-V5 region. Built from the Silva 138.1 SSU Ref NR 99 database using Qiime2 (version 2021.2).
Primers used:
EMP 16S 515f: GTGYCAGCMGCCGCGGTAA
EMP 16S 926r: CCGYCAATTYMTTTRAGTTT
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Notes
Files
Files
(266.3 MB)
Name | Size | Download all |
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md5:6efa6a3be132e928524fa0d317161995
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142.2 MB | Download |
md5:fbb7ab003ef4ada9e263367d1f30e640
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20.2 MB | Download |
md5:de8886bb2c059b1e8752255d271f3010
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97.1 MB | Download |
md5:f12d5b78bf4b1519721fe52803581c3d
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6.9 MB | Download |
Additional details
References
- Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucl. Acids Res. 41 (D1): D590-D596.
- Yilmaz P, Parfrey LW, Yarza P, Gerken J, Pruesse E, Quast C, Schweer T, Peplies J, Ludwig W, Glöckner FO (2014) The SILVA and "All-species Living Tree Project (LTP)" taxonomic frameworks. Nucl. Acids Res. 42:D643-D648
- Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R, Huttley GA, Caporaso JG. 2018. Optimizing taxonomic classification of marker gene sequences. Microbiome 6(1): 90. doi: https://doi.org/10.1186/s40168-018-0470-z.